Abstract：
Persistent organic pollutants (POPs) pose a significant global threat to human health and the environment, and require continuous monitoring due to their ability to migrate long distances. Active biomonitoring using cloned mosses is an inexpensive but underexplored method to assess POPs, mainly due to the poor understanding of the loading mechanisms of these pollutants in mosses. In this work, Fontinalis antipyretica (aquatic moss) and Sphagnum palustre (terrestrial moss) were evaluated as potential biomonitors of hexachlorocyclohexanes (HCHs: α-, β-, γ-, δ-HCH), crucial POPs. Moss clones, grown in photobioreactors and subsequently oven-dried, were used. Their lipid composition and distribution were characterized through molecular and histochemical studies. Adsorption experiments were carried out in the aqueous phase using the repeated additions method and in the gas phase using an active air sampling technique based on solid-phase extraction, a pioneering approach in moss research. F. antipyretica exhibited greater lipid content in the walls of most cells and higher adsorption capacity for all HCH isomers in both gaseous and liquid environments. These findings highlight the need for further investigation of POP loading mechanisms in mosses and open the door to explore other species based on their lipid content.
摘要：
持久性有机污染物(POPs)对人类健康和环境构成重大的全球性威胁,并且由于它们能够长距离迁移，因此需要连续监控。使用克隆苔藓进行主动生物监测是一种廉价但未充分开发的评估持久性有机污染物的方法，主要是由于对苔藓中这些污染物的负载机制了解不足。在这项工作中,Fontinalisantipyretica（水生苔藓）和Sphagnumpalustre（陆生苔藓）被评估为六氯环己烷的潜在生物监测器（HCHs：α-，β-,γ-,δ-HCH)，至关重要的持久性有机污染物。Moss克隆人,在光生物反应器中生长，随后烘干，被使用。通过分子和组织化学研究表征了它们的脂质组成和分布。在水相中使用重复添加方法进行吸附实验，在气相中使用基于固相萃取的主动空气采样技术进行吸附实验，苔藓研究的开创性方法。解热杆菌在大多数细胞壁中的脂质含量更高，在气态和液态环境中对所有六氯环己烷异构体的吸附能力更高。这些发现强调了进一步研究苔藓中POP负载机制的必要性，并为探索其他物种的脂质含量打开了大门。
