UNASSIGNED: Whole genome sequencing data of 32 E. coli strains collected from the feces of wild Amur tiger (Panthera tigris altaica, n = 24) and North China leopard (Panthera pardus japonensis, n = 8) were analyzed in this article. The multi-locus sequence types, serotypes, virulence and resistance genotypes, plasmid replicon types, and core genomic SNPs phylogeny of these isolates were studied. Additionally, antimicrobial susceptibility testing (AST) was performed on these E. coli isolates.
UNASSIGNED: Among the E. coli isolates studied, 18 different sequence types were identified, with ST939 (21.9%), ST10 (15.6%), and ST3246 (9.4%) being the most prevalent. A total of 111 virulence genes were detected, averaging about 54 virulence genes per sample. They contribute to invasion, adherence, immune evasion, efflux pump, toxin, motility, stress adaption, and other virulence-related functions of E. coli. Sixty-eight AMR genes and point mutations were identified. Among the detected resistance genes, those belonging to the efflux pump family were the most abundant. Thirty-two E. coli isolates showed the highest rate of resistance to tetracycline (14/32; 43.8%), followed by imipenem (4/32; 12.5%), ciprofloxacin (3/32; 9.4%), doxycycline (2/32; 6.3%), and norfloxacin (1/32; 3.1%).
UNASSIGNED: Our results suggest that E. coli isolates carried by wild Amur tigers and North China leopards have potential pathogenicity and drug resistance.
■从野生阿穆尔虎(Pantheratigrisaltaica,n=24)和华北豹(Pantherapardusjaponensis,n=8)进行了分析。多位点序列类型,血清型,毒力和抗性基因型,质粒复制子类型,并研究了这些分离株的核心基因组SNP系统发育。此外,对这些大肠杆菌分离株进行了抗菌药物敏感性试验(AST).
■在研究的大肠杆菌分离株中,鉴定出18种不同的序列类型,ST939(21.9%),ST10(15.6%),和ST3246(9.4%)是最普遍的。共检测到111个毒力基因,平均每个样本约54个毒力基因。它们有助于入侵,坚持,免疫逃避,外排泵,毒素,运动性,应力适应,和大肠杆菌的其他毒力相关功能。鉴定了68个AMR基因和点突变。在检测到的抗性基因中,那些属于外排泵家族的是最丰富的。32株大肠杆菌对四环素耐药率最高(14/32;43.8%),其次是亚胺培南(4/32;12.5%),环丙沙星(3/32;9.4%),多西环素(2/32;6.3%),和诺氟沙星(1/32;3.1%)。
■我们的结果表明,野生东北虎和华北豹携带的大肠杆菌分离株具有潜在的致病性和耐药性。