PDF(Pubmed)
Abstract：
The Australian skink Egernia stokesii had been recognised as a host of two species of Plasmodium, Plasmodium mackerrasae and P. circularis; nevertheless, molecular data are available for only a single haemosporidian species of this host. Its sequences are labelled as \"Plasmodium sp.\" or \"Plasmodium mackerrasae\", but morphological characteristics of this isolate are unavailable. Phylogenetic analyses of these sequences placed them into the clade of the genus Haemocystidium. In this study, blood samples of six E. stokesii were analysed by both, molecular and microscopic methods to clarify the haemosporidia of this lizard. Application of these approaches offered discordant results. Whereas sequence analysis clustered our isolates with lizard species of Haemocystidium, morphology of blood stages is more akin to Plasmodium than Haemocystidium. However, limited sampling, indistinguishable nuclei/merozoites and risk of possible hidden presence of mixed infection prevent reliable species identification of detected parasites or their description as new species of Haemocystidium.
摘要：
澳大利亚石龙子Egerniastokesii被认为是两种疟原虫的宿主，疟原虫和环状疟原虫；尽管如此，分子数据仅适用于该宿主的单个血孢菌物种。它的序列被标记为“疟原虫。\"或\"mackerrasae\"，但该分离株的形态特征不可用。对这些序列的系统发育分析将它们置于血细胞杆菌属的进化枝中。在这项研究中,两者都分析了六个E.stokesii的血液样本，分子和显微镜方法来阐明这种蜥蜴的血球。这些方法的应用提供了不一致的结果。而序列分析将我们的分离株与蜥蜴类血球属聚集在一起，血液阶段的形态更类似于疟原虫，而不是血球藻。然而,有限的采样，无法区分的核/裂殖子和可能隐藏的混合感染的风险阻止了对检测到的寄生虫的可靠物种鉴定或将其描述为新的血球菌种。
