可怕的Heloderma可怕的,一种原产于美国的有毒爬行动物,具有在治疗II型糖尿病中具有潜在应用的毒液。在这项工作中,H.H.提取了可怕的毒液,冻干,并使用透明质酸酶的酶测定法进行表征,磷脂酶,和蛋白酶。毒液的蛋白质组学分析采用自下而上/鸟枪法进行,SDS-PAGE,高pH反相色谱,并使用纳米LC-MS/MS分离胰蛋白酶肽。根据先前报道的转录组分类,对H.h.hordum毒液中发现的蛋白质进行了审查。蛋白质组学方法鉴定了101种酶,36种蛋白质,15种蛋白质抑制剂,11个宿主防御蛋白,和1种毒素,包括新的毒液成分,如钙结合蛋白,磷脂酶A2抑制剂,Serpins,组织蛋白酶,枯草杆菌酶,羧肽酶样,氨肽酶,糖苷水解酶,硫氧还蛋白转移酶,酸性神经酰胺酶样,烯醇化酶,多铜氧化酶,磷酸葡萄糖异构酶(PGI),果糖-1,6-双磷酸酶1类,五聚素相关,肽基甘氨酸α-羟基化单加氧酶/肽基-羟基甘氨酸α-酰胺化裂解酶,碳酸酐酶,乙酰胆碱酯酶,二肽基肽酶,和溶菌酶.这些发现有助于理解H.h.hordum的有毒性质,并强调其作为生物活性化合物来源的潜力。可通过PRoteomeXchange获得具有标识符PXD052417的数据。
Heloderma horridum horridum, a venomous reptile native to America, has a venom with potential applications in treating type II diabetes. In this work, H. h. horridum venom was extracted, lyophilized, and characterized using enzymatic assays for hyaluronidase, phospholipase, and protease. Proteomic analysis of the venom was conducted employing bottom-up/shotgun approaches, SDS-PAGE, high-pH reversed-phase chromatography, and fractionation of tryptic peptides using nano-LC-MS/MS. The proteins found in H. h. horridum venom were reviewed according to the classification of the transcriptome previously reported. The proteomic approach identified 101 enzymes, 36 other proteins, 15 protein inhibitors, 11 host defense proteins, and 1 toxin, including novel venom components such as calcium-binding proteins, phospholipase A2 inhibitors, serpins, cathepsin, subtilases, carboxypeptidase-like, aminopeptidases, glycoside hydrolases, thioredoxin transferases, acid ceramidase-like, enolase, multicopper oxidases, phosphoglucose isomerase (PGI), fructose-1,6-bisphosphatase class 1, pentraxin-related, peptidylglycine α-hydroxylating monooxygenase/peptidyl-hydroxyglycine α-amidating lyase, carbonic anhydrase, acetylcholinesterase, dipeptidylpeptidase, and lysozymes. These findings contribute to understanding the venomous nature of H. h. horridum and highlight its potential as a source of bioactive compounds. Data are available via PRoteomeXchange with the identifier PXD052417.