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Abstract：
Tracheal pooling for Mycoplasma hyopneumoniae (M. hyopneumoniae) DNA detection allows for decreased diagnostic cost, one of the main constraints in surveillance programs. The objectives of this study were to estimate the sensitivity of pooled-sample testing for the detection of M. hyopneumoniae in tracheal samples and to develop probability of M. hyopneumoniae detection estimates for tracheal samples pooled by 3, 5, and 10. A total of 48 M. hyopneumoniae PCR-positive field samples were pooled 3-, 5-, and 10-times using field M. hyopneumoniae DNA-negative samples and tested in triplicate. The sensitivity was estimated at 0.96 (95% credible interval [Cred. Int.]: 0.93, 0.98) for pools of 3, 0.95 (95% Cred. Int: 0.92, 0.98) for pools of 5, and 0.93 (95% Cred. Int.: 0.89, 0.96) for pools of 10. All pool sizes resulted in PCR-positive if the individual tracheal sample Ct value was < 33. Additionally, there was no significant decrease in the probability of detecting at least one M. hyopneumoniae-infected pig given any pool size (3, 5, or 10) of tracheal swabs. Furthermore, this manuscript applies the probability of detection estimates to various real-life diagnostic testing scenarios. Combining increased total animals sampled with pooling can be a cost-effective tool to maximize the performance of M. hyopneumoniae surveillance programs.
摘要：
猪肺炎支原体的气管汇集（M.猪肺炎)DNA检测可以降低诊断成本，监视计划的主要限制之一。本研究的目的是评估合并样本检测气管样本中猪肺炎支原体的灵敏度，并开发合并3、5和10的气管样本的猪肺炎支原体检测估计概率。共收集48例猪肺炎支原体PCR阳性现场样品3，5-,使用猪肺炎支原体DNA阴性样品10次，一式三份进行检测。敏感性估计为0.96(95%可信区间[Cred。Int.]：0.93，0.98），用于3，0.95（95%Cred。Int：0.92，0.98），用于5和0.93（95％Cred。Int.：0.89，0.96）对于10个池。如果单个气管样品Ct值<33,则所有池大小导致PCR阳性。此外,考虑到任何大小(3,5或10)的气管拭子池,检测到至少一头猪肺炎支原体感染的可能性均无显著下降.此外，本手稿将检测概率估计值应用于各种现实生活中的诊断测试场景.将增加的总动物采样与合并相结合可以是一种成本有效的工具，以最大化猪肺炎支原体监测计划的性能。
