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Abstract：
It has become increasingly evident that the structures RNAs adopt are conformationally dynamic; the various structured states that RNAs sample govern their interactions with other nucleic acids, proteins, and ligands to regulate a myriad of biological processes. Although several biophysical approaches have been developed and used to study the dynamic landscape of structured RNAs, technical limitations have limited their application to all classes of RNA due to variable size and flexibility. Recent advances combining chemical probing experiments with next-generation- and direct sequencing have emerged as an alternative approach to exploring the conformational dynamics of RNA. In this review, we provide a methodological overview of the sequencing-based techniques used to study RNA conformational dynamics. We discuss how different techniques have enabled us to better understand the propensity of RNAs from a variety of different classes to sample multiple conformational states. Finally, we present examples of the ways these techniques have reshaped how we think about RNA structure.
摘要：
越来越明显的是，RNA采用的结构是构象动态的；RNA样品的各种结构状态控制着它们与其他核酸的相互作用，蛋白质，和配体来调节无数的生物过程。尽管已经开发了几种生物物理方法并用于研究结构化RNA的动态景观，由于可变的大小和灵活性，技术限制限制了它们在所有类型的RNA中的应用。将化学探测实验与下一代和直接测序相结合的最新进展已成为探索RNA构象动力学的替代方法。在这次审查中,我们提供了用于研究RNA构象动力学的基于测序的技术的方法学概述.我们讨论了不同的技术如何使我们能够更好地理解来自各种不同类别的RNA对多个构象状态进行采样的倾向。最后,我们提供了这些技术如何重塑我们对RNA结构的看法的例子。
