PDF(Pubmed)
Abstract：
Silymarin, derived from Silybum marianum, has been used in traditional medicine for various ailments. In this study, the cardioprotective and hepatoprotective effects of silymarin against paracetamol-induced oxidative stress were examined in 28 male Swiss Webster mice, divided into four groups and treated for 7 days (via the oral route) with (a) saline 1 mL/kg (control group), (b) saline 1 mL/kg + single dose of paracetamol 110 mg/kg on the 7th day; (c) silymarin 50 mg/kg; and (d) silymarin 50 mg/kg + single dose of paracetamol 110 mg/kg on the 7th day. In vitro and in vivo antioxidant activity together with liver enzyme activity were evaluated. Histopathological and immunohistochemical assessment was performed. Silymarin mitigated paracetamol-induced liver injury by reducing oxidative stress markers such as lipid peroxidation and restoring antioxidant enzyme activity. Silymarin treatment resulted in a significant decrease in liver enzyme levels. Reduced necrosis and inflammatory infiltrate in liver tissues of silymarin-treated groups were detected as well. Immunohistochemical analysis demonstrated reduced expression of inflammatory markers (COX2, iNOS) and oxidative stress marker (SOD2) in the liver tissues of the silymarin-treated groups. Similar trends were observed in cardiac tissue. These results suggest that silymarin exerts potent hepatoprotective and cardioprotective effects against paracetamol-induced oxidative stress, making it a promising therapeutic agent for liver and heart diseases associated with oxidative damage.
摘要：
水飞蓟素,源自水飞蓟草，已被用于各种疾病的传统医学。在这项研究中,在28只雄性SwissWebster小鼠中检查了水飞蓟素对扑热息痛诱导的氧化应激的心脏保护和肝脏保护作用，分为四组，用（a）1mL/kg盐水（对照组）治疗7天（通过口服途径），(b)盐水1mL/kg+在第7天的单剂量对乙酰氨基酚110mg/kg;(c)水飞蓟素50mg/kg;和(d)在第7天的水飞蓟素50mg/kg+单剂量对乙酰氨基酚110mg/kg。评估了体外和体内抗氧化活性以及肝酶活性。进行组织病理学和免疫组织化学评估。水飞蓟素通过减少氧化应激标志物如脂质过氧化和恢复抗氧化酶活性来减轻扑热息痛诱导的肝损伤。水飞蓟素治疗导致肝酶水平显着降低。还检测到水飞蓟素治疗组的肝组织中坏死和炎症浸润减少。免疫组织化学分析表明水飞蓟素处理组的肝组织中炎症标志物（COX2，iNOS）和氧化应激标志物（SOD2）的表达降低。在心脏组织中观察到类似的趋势。这些结果表明，水飞蓟素对扑热息痛诱导的氧化应激发挥有效的肝保护和心脏保护作用，使其成为与氧化损伤相关的肝脏和心脏疾病的有前途的治疗剂。
