Protective Efficacy of Lyophilized Vesicular Stomatitis Virus-Based Vaccines in Animal Model.

基于冻干水泡性口炎病毒的疫苗在动物模型中的保护作用。

• 影响指数: 16.126
• 发表时间: 2024 May
• 来源期刊: Emerg Infect Dis
• DOI: 10.3201/eid3005.231248
• PMID: 38666640
• 文章类型: Journal Article
• 中科院分区: Q1
• 方向: 医学
• 作者列表: Salawudeen A,Soule G,Tailor N,Klassen L,Audet J,Sloan A,Deschambault Y,Safronetz D

关键词: Ebola virus; Lassa virus; animal models; glycoproteins; guinea pigs; lyophilized; vaccines; vesicular stomatitis virus; viruses; zoonoses

Mesh: Animals; Guinea Pigs; Freeze Drying; Viral Vaccines / immunology administration & dosage; Vesicular Stomatitis / immunology prevention & control virology; Disease Models, Animal; Vesiculovirus / immunology genetics; Antibodies, Viral / immunology blood; Vaccine Efficacy; Vesicular stomatitis Indiana virus / immunology

Abstract

We evaluated the in vitro effects of lyophilization for 2 vesicular stomatitis virus-based vaccines by using 3 stabilizing formulations and demonstrated protective immunity of lyophilized/reconstituted vaccine in guinea pigs. Lyophilization increased stability of the vaccines, but specific vesicular stomatitis virus-based vaccines will each require extensive analysis to optimize stabilizing formulations.

摘要

我们通过使用3种稳定制剂评估了冻干对2种基于水泡性口炎病毒的疫苗的体外作用，并证明了冻干/重组疫苗在豚鼠中的保护性免疫力。冻干增加了疫苗的稳定性，但特定的基于水疱性口炎病毒的疫苗都需要进行广泛的分析以优化稳定制剂.