METHODS: This laboratory study investigated primary HGFs viability, metabolic activity, cytotoxicity, and apoptotic events on preformed metal crown discs, composite resin-coated wells, and monolithic zirconia fragments at 24, 48, and 72 h using the ApoTox-Glo Triplex assay. The PPCs were also immersed in 0.1% lactic acid, 0.2% phosphoric acid, or 10% citric acid for 7 days at 37 °C to reproduce conditions associated with dietary intake or gastric reflux. Samples were then subject to inductively coupled plasma optical emission spectrometry to quantitate the release of ions.
RESULTS: The viability of HGFs on stainless steel and CR significantly declined at 48 and 72 h, representing potential cytotoxicity (p < 0.05). Cytotoxicity of HGFs was also higher for stainless steel and ZR compared to control (p < 0.05). PMCs and ZR crowns gave minimal ion release. Meanwhile, significant quantities of metallic ions, including copper (Cu), iron (Fe), nickel (Ni), and zinc (Zn), were present in eluates from veneered-preformed metal crowns.
CONCLUSIONS: As PPCs can be exposed to highly acidic environments for many years, thus the release of metallic ions from V-PMCs should form the further investigation in future studies.
方法:本实验室研究调查了原发性HGFs的活力,代谢活动,细胞毒性,和预制金属冠盘上的凋亡事件,复合树脂涂层井,和使用ApoTox-GloTriplex测定在24、48和72小时的整体氧化锆片段。还将PPC浸入0.1%的乳酸中,0.2%磷酸,或10%柠檬酸在37°C下持续7天,以重现与饮食摄入或胃反流相关的条件。然后对样品进行电感耦合等离子体发射光谱法以定量离子的释放。
结果:在48和72h时,HGFs在不锈钢和CR上的活力显着下降,代表潜在的细胞毒性(p<0.05)。与对照相比,不锈钢和ZR的HGF的细胞毒性也更高(p<0.05)。PMC和ZR冠给出了最小的离子释放。同时,大量的金属离子,包括铜(Cu),铁(Fe),镍(Ni),和锌(Zn),存在于镶面预制金属冠的洗脱液中。
结论:由于PPC可以暴露在高酸性环境中多年,因此,金属离子从V-PMCs中的释放应在未来的研究中进一步研究。