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Abstract：
OBJECTIVE: To investigate the in vitro biocompatibility of human gingival fibroblasts with preformed paediatric crowns and resistance to acid exposure at levels that simulate the oral environment.
METHODS: This laboratory study investigated primary HGFs viability, metabolic activity, cytotoxicity, and apoptotic events on preformed metal crown discs, composite resin-coated wells, and monolithic zirconia fragments at 24, 48, and 72 h using the ApoTox-Glo Triplex assay. The PPCs were also immersed in 0.1% lactic acid, 0.2% phosphoric acid, or 10% citric acid for 7 days at 37 °C to reproduce conditions associated with dietary intake or gastric reflux. Samples were then subject to inductively coupled plasma optical emission spectrometry to quantitate the release of ions.
RESULTS: The viability of HGFs on stainless steel and CR significantly declined at 48 and 72 h, representing potential cytotoxicity (p < 0.05). Cytotoxicity of HGFs was also higher for stainless steel and ZR compared to control (p < 0.05). PMCs and ZR crowns gave minimal ion release. Meanwhile, significant quantities of metallic ions, including copper (Cu), iron (Fe), nickel (Ni), and zinc (Zn), were present in eluates from veneered-preformed metal crowns.
CONCLUSIONS: As PPCs can be exposed to highly acidic environments for many years, thus the release of metallic ions from V-PMCs should form the further investigation in future studies.
METHODS: This laboratory study investigated primary HGFs viability, metabolic activity, cytotoxicity, and apoptotic events on preformed metal crown discs, composite resin-coated wells, and monolithic zirconia fragments at 24, 48, and 72 h using the ApoTox-Glo Triplex assay. The PPCs were also immersed in 0.1% lactic acid, 0.2% phosphoric acid, or 10% citric acid for 7 days at 37 °C to reproduce conditions associated with dietary intake or gastric reflux. Samples were then subject to inductively coupled plasma optical emission spectrometry to quantitate the release of ions.
RESULTS: The viability of HGFs on stainless steel and CR significantly declined at 48 and 72 h, representing potential cytotoxicity (p < 0.05). Cytotoxicity of HGFs was also higher for stainless steel and ZR compared to control (p < 0.05). PMCs and ZR crowns gave minimal ion release. Meanwhile, significant quantities of metallic ions, including copper (Cu), iron (Fe), nickel (Ni), and zinc (Zn), were present in eluates from veneered-preformed metal crowns.
CONCLUSIONS: As PPCs can be exposed to highly acidic environments for many years, thus the release of metallic ions from V-PMCs should form the further investigation in future studies.
摘要：
目的：研究人牙龈成纤维细胞与预先形成的儿科牙冠的体外生物相容性以及在模拟口腔环境的水平下对酸暴露的抵抗力。
方法：本实验室研究调查了原发性HGFs的活力，代谢活动，细胞毒性，和预制金属冠盘上的凋亡事件，复合树脂涂层井，和使用ApoTox-GloTriplex测定在24、48和72小时的整体氧化锆片段。还将PPC浸入0.1％的乳酸中，0.2%磷酸，或10％柠檬酸在37°C下持续7天，以重现与饮食摄入或胃反流相关的条件。然后对样品进行电感耦合等离子体发射光谱法以定量离子的释放。
结果：在48和72h时，HGFs在不锈钢和CR上的活力显着下降，代表潜在的细胞毒性(p<0.05)。与对照相比,不锈钢和ZR的HGF的细胞毒性也更高(p<0.05)。PMC和ZR冠给出了最小的离子释放。同时,大量的金属离子，包括铜（Cu），铁(Fe),镍(Ni),和锌（Zn），存在于镶面预制金属冠的洗脱液中。
结论：由于PPC可以暴露在高酸性环境中多年，因此，金属离子从V-PMCs中的释放应在未来的研究中进一步研究。
方法：本实验室研究调查了原发性HGFs的活力，代谢活动，细胞毒性，和预制金属冠盘上的凋亡事件，复合树脂涂层井，和使用ApoTox-GloTriplex测定在24、48和72小时的整体氧化锆片段。还将PPC浸入0.1％的乳酸中，0.2%磷酸，或10％柠檬酸在37°C下持续7天，以重现与饮食摄入或胃反流相关的条件。然后对样品进行电感耦合等离子体发射光谱法以定量离子的释放。
结果：在48和72h时，HGFs在不锈钢和CR上的活力显着下降，代表潜在的细胞毒性(p<0.05)。与对照相比,不锈钢和ZR的HGF的细胞毒性也更高(p<0.05)。PMC和ZR冠给出了最小的离子释放。同时,大量的金属离子，包括铜（Cu），铁(Fe),镍(Ni),和锌（Zn），存在于镶面预制金属冠的洗脱液中。
结论：由于PPC可以暴露在高酸性环境中多年，因此，金属离子从V-PMCs中的释放应在未来的研究中进一步研究。
