{Reference Type}: Journal Article
{Title}: Biocompatibility and acid resistance of preformed crowns in children: an in vitro study.
{Author}: Hogerheyde T;Coates D;Walsh L;Zafar S;
{Journal}: Eur Arch Paediatr Dent
{Volume}: 25
{Issue}: 3
{Year}: 2024 Jun 25
暂无{DOI}: 10.1007/s40368-024-00898-3
{Abstract}: <B>OBJECTIVE: </B>To investigate the in vitro biocompatibility of human gingival fibroblasts with preformed paediatric crowns and resistance to acid exposure at levels that simulate the oral environment.<BR><B>METHODS: </B>This laboratory study investigated primary HGFs viability, metabolic activity, cytotoxicity, and apoptotic events on preformed metal crown discs, composite resin-coated wells, and monolithic zirconia fragments at 24, 48, and 72 h using the ApoTox-Glo Triplex assay. The PPCs were also immersed in 0.1% lactic acid, 0.2% phosphoric acid, or 10% citric acid for 7 days at 37 °C to reproduce conditions associated with dietary intake or gastric reflux. Samples were then subject to inductively coupled plasma optical emission spectrometry to quantitate the release of ions.<BR><B>RESULTS: </B>The viability of HGFs on stainless steel and CR significantly declined at 48 and 72 h, representing potential cytotoxicity (p < 0.05). Cytotoxicity of HGFs was also higher for stainless steel and ZR compared to control (p < 0.05). PMCs and ZR crowns gave minimal ion release. Meanwhile, significant quantities of metallic ions, including copper (Cu), iron (Fe), nickel (Ni), and zinc (Zn), were present in eluates from veneered-preformed metal crowns.<BR><B>CONCLUSIONS: </B>As PPCs can be exposed to highly acidic environments for many years, thus the release of metallic ions from V-PMCs should form the further investigation in future studies.