Abstract：
Tissue culture optimization protocols limit indica rice breeding. Such a challenge is vital because emergent techniques still rely on tissue culture methods and could allow the breeding of new varieties with higher production and toleration of adverse environmental effects caused by climate change. Genome editing technology, using CRISPR/Cas9, is a fast and precise method for accelerated plant breeding. It limited its use in indica subspecies because of the recalcitrant response to in vitro culture methods. This chapter describes a protocol for CRISPR/Cas9 editing in indica subspecies, specifically in the CR-5272 variety derived from parental lines IR-822, using Agrobacterium tumefaciens and biolistic transformation.
摘要：
组织培养优化方案限制了in稻育种。这种挑战是至关重要的，因为新兴技术仍然依赖于组织培养方法，并且可以育种具有更高的产量和对气候变化造成的不利环境影响的耐受性的新品种。基因组编辑技术,使用CRISPR/Cas9，是一种快速，精确的加速植物育种方法。由于对体外培养方法的顽固反应，它限制了其在in子亚种中的使用。本章介绍了在in子亚种中进行CRISPR/Cas9编辑的协议，特别是在来自亲本品系IR-822的CR-5272品种中，使用根癌农杆菌和生物射弹转化。
