Abstract：
Athletes increasingly engage in repeated sprint training consisting in repeated short all-out efforts interspersed by short recoveries. When performed in hypoxia (RSH), it may lead to greater training effects than in normoxia (RSN); however, the underlying molecular mechanisms remain unclear. This study aimed at elucidating the effects of RSH on skeletal muscle metabolic adaptations as compared to RSN. Sixteen healthy young men performed nine repeated sprint training sessions in either normoxia (FIO2 = 0.209, RSN, n = 7) or normobaric hypoxia (FIO2 = 0.136, RSH, n = 9). Before and after the training period, exercise performance was assessed by using repeated sprint ability (RSA) and Wingate tests. Vastus lateralis muscle biopsies were performed to investigate muscle metabolic adaptations using proteomics combined with western blot analysis. Similar improvements were observed in RSA and Wingate tests in both RSN and RSH groups. At the muscle level, RSN and RSH reduced oxidative phosphorylation protein content but triggered an increase in mitochondrial biogenesis proteins. Proteomics showed an increase in several S100A family proteins in the RSH group, among which S100A13 most strongly. We confirmed a significant increase in S100A13 protein by western blot in RSH, which was associated with increased Akt phosphorylation and its downstream targets regulating protein synthesis. Altogether our data indicate that RSH may activate an S100A/Akt pathway to trigger specific adaptations as compared to RSN.
摘要：
运动员越来越多地进行反复的冲刺训练，其中包括反复进行的短暂的全力以赴的努力，以及短暂的恢复。当在缺氧（RSH）中进行时，它可能导致更大的训练效果比在常氧(RSN);然而，潜在的分子机制尚不清楚.本研究旨在阐明与RSN相比RSH对骨骼肌代谢适应的影响。16名健康的年轻人在两种正常的情况下进行了9次重复的冲刺训练（FIO2=0.209，RSN，n=7)或常压缺氧(FIO2=0.136，RSH，n=9)。培训前后，通过重复冲刺能力（RSA）和Wingate测试评估运动表现。使用蛋白质组学结合蛋白质印迹分析进行股外侧肌活检以研究肌肉代谢适应。在RSN和RSH组的RSA和Wingate测试中观察到类似的改善。在肌肉水平，RSN和RSH降低了氧化磷酸化蛋白含量，但触发了线粒体生物发生蛋白的增加。蛋白质组学显示RSH组中几种S100A家族蛋白的增加，其中S100A13最强。我们通过蛋白质印迹证实了RSH中S100A13蛋白的显着增加，这与Akt磷酸化增加及其调节蛋白质合成的下游靶标有关。总之，我们的数据表明，与RSN相比，RSH可以激活S100A/Akt途径以触发特定的适应。
