泛菌栖息在不同的生态位,从植物的附生植物和内生菌,动物的身体,偶尔在人类系统中。这种多方面的细菌对促进植物生长有很大的贡献,应力弹性,和生物防治,但也可以作为其宿主的病原体。这些不同功能背后的遗传决定因素在很大程度上仍然是未知的,为了揭示这种现象,选择并分析了19株泛菌种。基因组到基因组距离计算器(GGDC)使用基因组爆炸距离系统发育(GBDP)技术来计算数字DDH值。通过基因组到基因组距离进行系统发育分析,平均核苷酸同一性,氨基酸鉴定计算表明,所有菌株都属于泛菌属。然而,菌株33.1的值低于相同物种划定的阈值。细菌全基因组分析(BPGA)管道和MinPath分析揭示了与环境韧性相关的遗传性状,如氧化应激,UV辐射,极端温度,和不同宿主特异性碳水化合物的代谢。蛋白质相互作用组分析说明渗透应激蛋白与核心蛋白密切相关,而重金属的耐受性,氮代谢,和通常与致病性相关的III型和VI型分泌系统蛋白形成了一个单独的网络,表明特定于菌株的特征。这些发现为泛菌复杂的遗传结构提供了新的启示,揭示了它在不同生态位的适应性,并在不同的环境中茁壮成长。
Pantoea agglomerans inhabit diverse ecological niches, ranging from epiphytes and endophytes in plants, body of animals, and occasionally in the human system. This multifaceted bacterium contributes substantially to plant growth promotion, stress resilience, and biocontrol but can also act as a pathogen to its host. The genetic determinants underlying these diverse functions remain largely unfathomed and to uncover this phenomenon, nineteen strains of Pantoea agglomerans were selected and analyzed. Genome-to-Genome Distance Calculator (GGDC) which uses the Genome Blast Distance Phylogeny (GBDP) technique to calculate digital DDH values. Phylogenetic analysis via Genome-to-Genome distance, Average Nucleotide Identity, and Amino Acid Identity calculation revealed that all strains belonged to the genus Pantoea. However, strain 33.1 had a lower value than the threshold for the same species delineation. Bacterial Pan Genome Analysis (BPGA) Pipeline and MinPath analysis revealed genetic traits associated with environmental resilience, such as oxidative stress, UV radiation, temperature extremes, and metabolism of distinct host-specific carbohydrates. Protein-protein interactome analysis illustrated osmotic stress proteins closely linked with core proteins, while heavy metal tolerance, nitrogen metabolism, and Type III and VI secretion systems proteins generally associated with pathogenicity formed a separate network, indicating strain-specific characteristics. These findings shed new light on the intricate genetic architecture of Pantoea agglomerans, revealing its adaptability to inhabit diverse niches and thrive in varied environments.