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Abstract：
The filamentous fungus Trichoderma reesei has been widely used for cellulase production that has extensive applications in green and sustainable development. Increasing costs and depletion of fossil fuels provoke the demand for hyper-cellulase production in this cellulolytic fungus. To better manipulate T. reesei for enhanced cellulase production and to lower the cost for large-scale fermentation, it is wise to have a comprehensive understanding of the crucial factors and complicated biological network of cellulase production that could provide new perspectives for further exploration and modification. In this review, we summarize recent progress and give an overview of the cellular process of cellulase production in T. reesei, including the carbon source-dependent cellulase induction, complicated transcriptional regulation network, and efficient protein assembly and trafficking. Among that, the key factors involved in cellulase production were emphasized, shedding light on potential perspectives for further engineering.
摘要：
丝状真菌里氏木霉已广泛用于纤维素酶生产，在绿色可持续发展中具有广泛的应用。不断增加的成本和化石燃料的消耗激发了这种纤维素分解真菌中对高纤维素酶生产的需求。为了更好地操纵里氏木霉，以提高纤维素酶的产量，并降低大规模发酵的成本，全面了解纤维素酶生产的关键因素和复杂的生物网络是明智的，这可以为进一步探索和改造提供新的视角。在这次审查中,我们总结了最近的进展，并概述了里氏木霉纤维素酶生产的细胞过程，包括碳源依赖性纤维素酶诱导，复杂的转录调控网络，以及高效的蛋白质组装和贩运。其中,强调了纤维素酶生产中涉及的关键因素，为进一步的工程提供潜在的前景。
