PDF(Pubmed)
Abstract：
The expansion of agriculture and the need for sustainable practices drives breeders to develop plant varieties better adapted to abiotic stress such as nutrient deficiency, which negatively impacts yields. Phosphorus (P) is crucial for photosynthesis and plant growth, but its availability in the soil is often limited, hampering crop development. In this study, we examined the response of two popcorn inbred lines, L80 and P7, which have been characterized previously as P-use inefficient and P-use efficient, respectively, under low (stress) and high P (control) availability. Physiological measurements, proteomic analysis, and metabolite assays were performed to unravel the physiological and molecular responses associated with the efficient use of P in popcorn. We observed significant differences in protein abundances in response to the P supply between the two inbred lines. A total of 421 differentially expressed proteins (DEPs) were observed in L80 and 436 DEPs in P7. These proteins were involved in photosynthesis, protein biosynthesis, biosynthesis of secondary metabolites, and energy metabolism. In addition, flavonoids accumulated in higher abundance in P7. Our results help us understand the major components of P utilization in popcorn, providing new insights for popcorn molecular breeding programs.
摘要：
农业的扩展和对可持续做法的需求促使育种者开发更适应非生物胁迫的植物品种，例如营养缺乏，这会对产量产生负面影响。磷（P）对光合作用和植物生长至关重要，但是它在土壤中的可用性通常是有限的，阻碍作物发育。在这项研究中,我们检查了两个爆米花近交系的反应，L80和P7，以前被描述为P使用效率低和P使用效率高，分别,在低（压力）和高P（控制）可用性下。生理测量,蛋白质组学分析，并进行了代谢物测定，以阐明与爆米花中P的有效利用相关的生理和分子反应。我们观察到两个自交系之间响应P供应的蛋白质丰度存在显着差异。在L80和P7中的436个DEP中观察到总共421个差异表达的蛋白质(DEP)。这些蛋白质参与光合作用，蛋白质生物合成，次级代谢产物的生物合成，和能量代谢。此外,类黄酮在P7中积累的丰度较高。我们的结果有助于我们了解爆米花中磷利用的主要成分，为爆米花分子育种计划提供新的见解。
