PDF(Pubmed)
Abstract：
UNASSIGNED: Bacterial infections causing digestive problems are among the most serious threats to Egypt\'s duck industry, owing to their effects on feed utilization and body weight gain.
UNASSIGNED: As a result, the goal of this study was to identify bacterial pathogens causing enteritis in ducks as well as testing their antimicrobials resistance capabilities.
UNASSIGNED: Forty-two duck flocks from different localities at four Egyptian Governorates (El-Sharkia, El-Gharbia, El-Dakahlia, and El-Qaliobia) have been subjected to clinical and postmortem examination as well as bacterial isolation and identification. The liver samples have been collected aseptically from freshly euthanized ducks for bacterial isolation followed by identification using conventional biochemical tests, VITEK 2 system, and confirmatory polymerase chain reaction (PCR) for detection of the uid A gene (beta-glucuronidase enzyme) of Escherichia coli. In addition, antimicrobial sensitivity testing for the isolates against different antimicrobials by the VITEK 2 system was used.
UNASSIGNED: Forty-six positive bacterial isolates were identified using conventional methods and the VITEK 2 system including Staphylococcus spp. (52.17%), E. coli (41.30%), and 2.17% for each of Enterococcus casseli lavus, Salmonella enterica subspecies arizonae, and Enterobacter cloacae. PCR was positive for E. coli uid A gene at 556 bp. The antibiogram patterns of isolated pathogens from naturally infected ducks in our work demonstrated 87% multidrug resistance with varying results against different antimicrobial drugs tested. Such findings supported the fact of the upgrading multidrug resistance of Staphylococci and Enterobacteriacae.
UNASSIGNED: The most prevalent bacterial pathogens associated with duck enteritis were Staphylococcus spp. and E. coli with the first report of S. enterica subspecies arizonae causing duck enteritis in Egypt.
UNASSIGNED: As a result, the goal of this study was to identify bacterial pathogens causing enteritis in ducks as well as testing their antimicrobials resistance capabilities.
UNASSIGNED: Forty-two duck flocks from different localities at four Egyptian Governorates (El-Sharkia, El-Gharbia, El-Dakahlia, and El-Qaliobia) have been subjected to clinical and postmortem examination as well as bacterial isolation and identification. The liver samples have been collected aseptically from freshly euthanized ducks for bacterial isolation followed by identification using conventional biochemical tests, VITEK 2 system, and confirmatory polymerase chain reaction (PCR) for detection of the uid A gene (beta-glucuronidase enzyme) of Escherichia coli. In addition, antimicrobial sensitivity testing for the isolates against different antimicrobials by the VITEK 2 system was used.
UNASSIGNED: Forty-six positive bacterial isolates were identified using conventional methods and the VITEK 2 system including Staphylococcus spp. (52.17%), E. coli (41.30%), and 2.17% for each of Enterococcus casseli lavus, Salmonella enterica subspecies arizonae, and Enterobacter cloacae. PCR was positive for E. coli uid A gene at 556 bp. The antibiogram patterns of isolated pathogens from naturally infected ducks in our work demonstrated 87% multidrug resistance with varying results against different antimicrobial drugs tested. Such findings supported the fact of the upgrading multidrug resistance of Staphylococci and Enterobacteriacae.
UNASSIGNED: The most prevalent bacterial pathogens associated with duck enteritis were Staphylococcus spp. and E. coli with the first report of S. enterica subspecies arizonae causing duck enteritis in Egypt.
摘要：
引起消化问题的细菌感染是埃及养鸭业最严重的威胁之一，由于它们对饲料利用和体重增加的影响。
■因此，这项研究的目的是鉴定引起鸭肠炎的细菌病原体，并测试其对抗菌药物的耐药性。
■来自四个埃及省不同地区的42只鸭子群（El-Sharkia，El-Gharbia,El-Dakahlia,和El-Qaliobia）已接受临床和尸检以及细菌分离和鉴定。从新鲜安乐死的鸭中无菌收集肝脏样本，用于细菌分离，然后使用常规生化测试进行鉴定，VITEK2系统,和用于检测大肠杆菌uidA基因（β-葡糖醛酸酶）的确证聚合酶链反应（PCR）。此外,使用VITEK2系统对分离株对不同抗菌药物的抗菌敏感性测试.
■使用常规方法和包括葡萄球菌属在内的VITEK2系统鉴定了46个阳性细菌分离株。（52.17%），大肠杆菌（41.30%），卡塞利蓝藻肠球菌各占2.17%，肠炎沙门氏菌亚种，阴沟肠杆菌.556bp的大肠杆菌uidA基因PCR呈阳性。在我们的工作中，从自然感染的鸭子中分离出的病原体的抗菌谱模式证明了87％的多药耐药性，对测试的不同抗菌药物的结果各不相同。这些发现支持了葡萄球菌和肠杆菌的多药耐药性升级的事实。
■与鸭肠炎相关的最常见的细菌病原体是葡萄球菌。和大肠杆菌，在埃及首次报道肠沙门氏菌亚种引起鸭肠炎。
■因此，这项研究的目的是鉴定引起鸭肠炎的细菌病原体，并测试其对抗菌药物的耐药性。
■来自四个埃及省不同地区的42只鸭子群（El-Sharkia，El-Gharbia,El-Dakahlia,和El-Qaliobia）已接受临床和尸检以及细菌分离和鉴定。从新鲜安乐死的鸭中无菌收集肝脏样本，用于细菌分离，然后使用常规生化测试进行鉴定，VITEK2系统,和用于检测大肠杆菌uidA基因（β-葡糖醛酸酶）的确证聚合酶链反应（PCR）。此外,使用VITEK2系统对分离株对不同抗菌药物的抗菌敏感性测试.
■使用常规方法和包括葡萄球菌属在内的VITEK2系统鉴定了46个阳性细菌分离株。（52.17%），大肠杆菌（41.30%），卡塞利蓝藻肠球菌各占2.17%，肠炎沙门氏菌亚种，阴沟肠杆菌.556bp的大肠杆菌uidA基因PCR呈阳性。在我们的工作中，从自然感染的鸭子中分离出的病原体的抗菌谱模式证明了87％的多药耐药性，对测试的不同抗菌药物的结果各不相同。这些发现支持了葡萄球菌和肠杆菌的多药耐药性升级的事实。
■与鸭肠炎相关的最常见的细菌病原体是葡萄球菌。和大肠杆菌，在埃及首次报道肠沙门氏菌亚种引起鸭肠炎。
