Abstract：
BACKGROUND: Ethylene diamine tetra-acetic acid (EDTA) is a chelating agent used to dissolve calcium deposits but evidence in decalcifying atherosclerotic lesions is limited.
OBJECTIVE: We assessed the feasibility and efficacy of EDTA delivered via porous balloon to target calcified lesions in cadaveric below-the-knee (BTK) arteries.
METHODS: Using porcine carotid arteries, EDTA concentration was measured in the arterial wall and outside the artery at the 0-, 0.5-, 4-, and 24-h circulation after the injection through a porous balloon. In cadaver BTK samples, the proximal and distal anterior tibial artery (ATA) and distal posterior tibial artery (PTA) were studied. EDTA-2Na/H2O or EDTA-3Na/H2O were administrated using a porous balloon, then circulated for 6 h for EDTA-3Na/H2O and 24 h for EDTA-2Na/H2O and EDTA-3Na/H2O. Micro-CT imaging of the artery segments before and after the circulation and cross-sectional analyses were performed to evaluate calcium burden.
RESULTS: In the porcine carotid study, EDTA was delivered through a porous balloon present in the arterial wall and was retained there for 24 h. In BTK arteries, cross-sectional analyses of micro-CT revealed a significant decrease in the calcium area in the distal ATA segment under 24-h circulation with EDTA-2Na/H2O and in the distal ATA segment under 24-h circulation with EDTA-3Na/H2O. The proximal ATA segment under 6-h circulation with EDTA-3Na/H2O showed no significant change in any parameters of calcium CONCLUSION: EDTA-3Na/H2O or EDTA-2Na/H2O with longer circulation times resulted in greater calcium reduction in atherosclerotic lesion. EDTA may have a potential therapeutic option for the treatment of atherosclerotic calcified lesions.
OBJECTIVE: We assessed the feasibility and efficacy of EDTA delivered via porous balloon to target calcified lesions in cadaveric below-the-knee (BTK) arteries.
METHODS: Using porcine carotid arteries, EDTA concentration was measured in the arterial wall and outside the artery at the 0-, 0.5-, 4-, and 24-h circulation after the injection through a porous balloon. In cadaver BTK samples, the proximal and distal anterior tibial artery (ATA) and distal posterior tibial artery (PTA) were studied. EDTA-2Na/H2O or EDTA-3Na/H2O were administrated using a porous balloon, then circulated for 6 h for EDTA-3Na/H2O and 24 h for EDTA-2Na/H2O and EDTA-3Na/H2O. Micro-CT imaging of the artery segments before and after the circulation and cross-sectional analyses were performed to evaluate calcium burden.
RESULTS: In the porcine carotid study, EDTA was delivered through a porous balloon present in the arterial wall and was retained there for 24 h. In BTK arteries, cross-sectional analyses of micro-CT revealed a significant decrease in the calcium area in the distal ATA segment under 24-h circulation with EDTA-2Na/H2O and in the distal ATA segment under 24-h circulation with EDTA-3Na/H2O. The proximal ATA segment under 6-h circulation with EDTA-3Na/H2O showed no significant change in any parameters of calcium CONCLUSION: EDTA-3Na/H2O or EDTA-2Na/H2O with longer circulation times resulted in greater calcium reduction in atherosclerotic lesion. EDTA may have a potential therapeutic option for the treatment of atherosclerotic calcified lesions.
摘要：
背景：乙二胺四乙酸（EDTA）是一种用于溶解钙沉积物的螯合剂，但动脉粥样硬化病变脱钙的证据有限。
目的：我们评估了通过多孔球囊递送EDTA靶向尸体膝下动脉（BTK）钙化病变的可行性和有效性。
方法：使用猪颈动脉，在0-0处测量动脉壁中和动脉外的EDTA浓度，0.5-,4-,以及通过多孔球囊注射后24小时循环。在尸体BTK样本中，研究了胫骨前动脉（ATA）和胫骨后动脉（PTA）的近端和远端。使用多孔球囊施用EDTA-2Na/H2O或EDTA-3Na/H2O，然后对于EDTA-3Na/H2O循环6小时,对于EDTA-2Na/H2O和EDTA-3Na/H2O循环24小时。进行循环前后动脉段的Micro-CT成像和横截面分析以评估钙负荷。
结果：在猪颈动脉研究中，EDTA通过存在于动脉壁中的多孔球囊递送，并在那里保留24小时。在BTK动脉中，显微CT的横截面分析表明，在使用EDTA-2Na/H2O的24小时循环下，ATA远端段的钙面积显着减少，而在使用EDTA-3Na/H2O的24小时循环下，ATA远端段的钙面积显着减少。在使用EDTA-3Na/H2O的6小时循环下，近端ATA节段在钙的任何参数中都没有显着变化。结论：循环时间较长的EDTA-3Na/H2O或EDTA-2Na/H2O导致动脉粥样硬化病变中钙的减少更大。EDTA可能具有治疗动脉粥样硬化钙化病变的潜在治疗选择。
目的：我们评估了通过多孔球囊递送EDTA靶向尸体膝下动脉（BTK）钙化病变的可行性和有效性。
方法：使用猪颈动脉，在0-0处测量动脉壁中和动脉外的EDTA浓度，0.5-,4-,以及通过多孔球囊注射后24小时循环。在尸体BTK样本中，研究了胫骨前动脉（ATA）和胫骨后动脉（PTA）的近端和远端。使用多孔球囊施用EDTA-2Na/H2O或EDTA-3Na/H2O，然后对于EDTA-3Na/H2O循环6小时,对于EDTA-2Na/H2O和EDTA-3Na/H2O循环24小时。进行循环前后动脉段的Micro-CT成像和横截面分析以评估钙负荷。
结果：在猪颈动脉研究中，EDTA通过存在于动脉壁中的多孔球囊递送，并在那里保留24小时。在BTK动脉中，显微CT的横截面分析表明，在使用EDTA-2Na/H2O的24小时循环下，ATA远端段的钙面积显着减少，而在使用EDTA-3Na/H2O的24小时循环下，ATA远端段的钙面积显着减少。在使用EDTA-3Na/H2O的6小时循环下，近端ATA节段在钙的任何参数中都没有显着变化。结论：循环时间较长的EDTA-3Na/H2O或EDTA-2Na/H2O导致动脉粥样硬化病变中钙的减少更大。EDTA可能具有治疗动脉粥样硬化钙化病变的潜在治疗选择。
