PDF(Pubmed)
Abstract：
UNASSIGNED: Full-field optical coherence microscopy (FF-OCM) is a prevalent technique for backscattering and phase imaging with epi-detection. Traditional methods have two limitations: suboptimal utilization of functional information about the sample and complicated optical design with several moving parts for phase contrast.
UNASSIGNED: We report an OCM setup capable of generating dynamic intensity, phase, and pseudo-spectroscopic contrast with single-shot full-field video-rate imaging called bichromatic tetraphasic (BiTe) full-field OCM with no moving parts.
UNASSIGNED: BiTe OCM resourcefully uses the phase-shifting properties of anti-reflection (AR) coatings outside the rated bandwidths to create four unique phase shifts, which are detected with two emission filters for spectroscopic contrast.
UNASSIGNED: BiTe OCM overcomes the disadvantages of previous FF-OCM setup techniques by capturing both the intensity and phase profiles without any artifacts or speckle noise for imaging scattering samples in three-dimensional (3D). BiTe OCM also utilizes the raw data effectively to generate three complementary contrasts: intensity, phase, and color. We demonstrate BiTe OCM to observe cellular dynamics, image live, and moving micro-animals in 3D, capture the spectroscopic hemodynamics of scattering tissues along with dynamic intensity and phase profiles, and image the microstructure of fall foliage with two different colors.
UNASSIGNED: BiTe OCM can maximize the information efficiency of FF-OCM while maintaining overall simplicity in design for quantitative, dynamic, and spectroscopic characterization of biological samples.
UNASSIGNED: We report an OCM setup capable of generating dynamic intensity, phase, and pseudo-spectroscopic contrast with single-shot full-field video-rate imaging called bichromatic tetraphasic (BiTe) full-field OCM with no moving parts.
UNASSIGNED: BiTe OCM resourcefully uses the phase-shifting properties of anti-reflection (AR) coatings outside the rated bandwidths to create four unique phase shifts, which are detected with two emission filters for spectroscopic contrast.
UNASSIGNED: BiTe OCM overcomes the disadvantages of previous FF-OCM setup techniques by capturing both the intensity and phase profiles without any artifacts or speckle noise for imaging scattering samples in three-dimensional (3D). BiTe OCM also utilizes the raw data effectively to generate three complementary contrasts: intensity, phase, and color. We demonstrate BiTe OCM to observe cellular dynamics, image live, and moving micro-animals in 3D, capture the spectroscopic hemodynamics of scattering tissues along with dynamic intensity and phase profiles, and image the microstructure of fall foliage with two different colors.
UNASSIGNED: BiTe OCM can maximize the information efficiency of FF-OCM while maintaining overall simplicity in design for quantitative, dynamic, and spectroscopic characterization of biological samples.
摘要：
■全视场光学相干显微镜（FF-OCM）是一种用于背散射和具有外延检测的相位成像的流行技术。传统方法有两个局限性：关于样品的功能信息的次优利用和具有多个运动部件的复杂光学设计用于相衬。
■我们报告了一种能够产生动态强度的OCM设置，阶段,和伪光谱对比度与单发全场视频速率成像，称为双色四相（BiTe）全场OCM，没有移动部件。
■BiTeOCM在额定带宽之外资源地使用抗反射（AR）涂层的相移特性来创建四个独特的相移，用两个发射滤光片检测光谱对比度。
■BiTeOCM通过捕获强度和相位轮廓而没有任何伪影或斑点噪声，从而克服了先前FF-OCM设置技术的缺点，用于对三维（3D）中的散射样品进行成像。BiTeOCM还有效地利用原始数据来生成三个互补对比：强度，阶段,和颜色。我们展示了BiTeOCM来观察细胞动力学，图像生活，在3D中移动微型动物，捕获散射组织的光谱血液动力学以及动态强度和相位曲线，并用两种不同的颜色对秋叶的微观结构进行成像。
■BiTeOCM可以最大限度地提高FF-OCM的信息效率，同时保持定量设计的整体简单性，动态,和生物样品的光谱表征。
■我们报告了一种能够产生动态强度的OCM设置，阶段,和伪光谱对比度与单发全场视频速率成像，称为双色四相（BiTe）全场OCM，没有移动部件。
■BiTeOCM在额定带宽之外资源地使用抗反射（AR）涂层的相移特性来创建四个独特的相移，用两个发射滤光片检测光谱对比度。
■BiTeOCM通过捕获强度和相位轮廓而没有任何伪影或斑点噪声，从而克服了先前FF-OCM设置技术的缺点，用于对三维（3D）中的散射样品进行成像。BiTeOCM还有效地利用原始数据来生成三个互补对比：强度，阶段,和颜色。我们展示了BiTeOCM来观察细胞动力学，图像生活，在3D中移动微型动物，捕获散射组织的光谱血液动力学以及动态强度和相位曲线，并用两种不同的颜色对秋叶的微观结构进行成像。
■BiTeOCM可以最大限度地提高FF-OCM的信息效率，同时保持定量设计的整体简单性，动态,和生物样品的光谱表征。
