{Reference Type}: Journal Article
{Title}: Bichromatic tetraphasic full-field optical coherence microscopy.
{Author}: Iyer RR;Žurauskas M;Rao Y;Chaney EJ;Boppart SA;
{Journal}: J Biomed Opt
{Volume}: 29
{Issue}: 0
{Year}: 2024 Jun
{Factor}: 3.758
{DOI}: 10.1117/1.JBO.29.S2.S22704
{Abstract}: <B>UNASSIGNED: </B>Full-field optical coherence microscopy (FF-OCM) is a prevalent technique for backscattering and phase imaging with epi-detection. Traditional methods have two limitations: suboptimal utilization of functional information about the sample and complicated optical design with several moving parts for phase contrast.<BR><B>UNASSIGNED: </B>We report an OCM setup capable of generating dynamic intensity, phase, and pseudo-spectroscopic contrast with single-shot full-field video-rate imaging called bichromatic tetraphasic (BiTe) full-field OCM with no moving parts.<BR><B>UNASSIGNED: </B>BiTe OCM resourcefully uses the phase-shifting properties of anti-reflection (AR) coatings outside the rated bandwidths to create four unique phase shifts, which are detected with two emission filters for spectroscopic contrast.<BR><B>UNASSIGNED: </B>BiTe OCM overcomes the disadvantages of previous FF-OCM setup techniques by capturing both the intensity and phase profiles without any artifacts or speckle noise for imaging scattering samples in three-dimensional (3D). BiTe OCM also utilizes the raw data effectively to generate three complementary contrasts: intensity, phase, and color. We demonstrate BiTe OCM to observe cellular dynamics, image live, and moving micro-animals in 3D, capture the spectroscopic hemodynamics of scattering tissues along with dynamic intensity and phase profiles, and image the microstructure of fall foliage with two different colors.<BR><B>UNASSIGNED: </B>BiTe OCM can maximize the information efficiency of FF-OCM while maintaining overall simplicity in design for quantitative, dynamic, and spectroscopic characterization of biological samples.