Abstract：
A bio-guided isolation was applied to the Vietnamese lichen Roccella montagnei based on alpha-glucosidase inhibition. Six compounds were isolated and structurally elucidated, including a new ortho depside, montagneside A (1), together with five known compounds, sekikaic acid (2), lanost-7-en-3β-ol (3), ethyl orsellinate (4), D-montagnetol (5), and D-erythrin (6). Their chemical structures were identified by extensive 1D and 2D NMR analysis, high-resolution mass spectroscopy, and comparisons with those reported in the literature. D-Erythrin (6), a major component, was selected for further modification using Smiles rearrangement. Three erythritol derivatives 6a-6c were synthesized. Compounds 1-3, 6, and 6a-6c were evaluated for alpha-glucosidase inhibition. Compounds 2 and 6a-6c showed significant alpha-glucosidase inhibition with IC50 values ranging from 7.9 to 149 μM, respectively. Molecular docking was applied to the most active compound 6a to clarify the inhibitory mechanism.
摘要：
基于α-葡萄糖苷酶的抑制作用，对越南地衣Roccellamontgnei进行了生物引导分离。分离并在结构上阐明了六个化合物，包括一个新的正交深度，蒙磁石A(1)，连同五种已知的化合物，己酸(2),lanost-7-en-3β-ol（3），orsellinate(4),D-montagnetol(5),和D-红霉素(6)。通过广泛的1D和2DNMR分析鉴定了它们的化学结构，高分辨率质谱,以及与文献报道的比较。D-Erythrin(6),一个主要组成部分，选择使用微笑重排进行进一步修改。合成了三种赤藓糖醇衍生物6a-6c。评价化合物1-3、6和6a-6c的α-葡糖苷酶抑制。化合物2和6a-6c显示出显着的α-葡萄糖苷酶抑制作用，IC50值为7.9至149µM，分别。将分子对接应用于最具活性的化合物6a以阐明抑制机制。
