染料木素(GEN)和甘氨酸(GLY),是主要从大豆植物中提取的异黄酮类型,尽管GEN具有更强的抗氧化和促进生长的作用。日粮GEN和GLY对繁殖性能的影响,鸡蛋质量,和骨质量在研究中进行了调查。此外,探索潜在的作用机制,研究了血清激素水平和生殖相关基因。总共378只Hy-Line棕色蛋鸡(120日龄)被随机分配到3个饮食组(对照组),(GLY,和GEN分别为50mg/kg),为期8周。每种处理具有126只鸟(每个18只鸟的7个重复)。结果分两个阶段进行分析:喂养试验的第1至4周和第5至8周。结果表明,补充GEN显着增加卵数,母鸡日产量(HDP),和第1周至第4周的鸡蛋质量,而,糖素和金雀异黄素都增加了卵子数量,鸡蛋重量,鸡蛋质量,在第5至8周期间,HDP和改善的饲料-鸡蛋比。鸡蛋质量分析显示蛋壳质量有显著改善;光泽度,厚度,力量,和蛋白质量指标(蛋白高度,Haugh单位,厚蛋白部分)由于饮食处理。此外,胫骨强度,胫骨灰分中的Ca含量和骨矿物质含量,通过饮食处理显着增加。血清E2、LH、FSH,T3、T4和GH,和抗氧化酶的活性;SOD,CAT,GSH在降低MDA水平的同时,值得注意的是治疗。此外,生殖相关基因:ESR1,FSHR,PRLR,GNRH1被GEN和GLY的补充显著上调。GEN相对于评估参数的功效优于GLY。最后,我们推测铺设性能的改善,鸡蛋质量和胫骨质量可能与异黄酮对钙代谢的促进作用有关,抗氧化功能,生殖激素和相关基因。因此,以50mg/kg的剂量水平补充GEN,可用于提高铺设性能,维持产蛋鸡的产蛋量和生理功能。
Genistein (GEN) and Glycitein (GLY), are types of isoflavone extracted mainly from soy plants, although GEN is associated with stronger antioxidant and growth-promoting effects. The impact of dietary GEN and GLY on reproductive performance, egg quality, and bone quality were investigated in the study. Additionally, to explore the underlying mechanism of action, the serum hormone levels and reproductive-related genes were investigated. A total of 378 Hy-Line Brown laying hens (120 days old) were randomly allocated to 3 dietary groups (Control), (GLY, and GEN at 50 mg/kg respectively) for a period of 8 wk. Each treatment has 126 birds (7 replicates of 18 birds each). Results were analyzed in 2 phases: wk 1 to 4, and 5 to 8 of feeding trial. The results indicated that supplemental GEN significantly increased egg number, hen-day production (HDP), and egg mass during wk 1 to 4, whereas, both glycitein and genistein increased egg number, egg weight, egg mass, HDP and improved feed-egg-ratio during wk 5 to 8. Egg quality analysis revealed significant improvements in eggshell quality; gloss, thickness, strength, and albumen quality indices (albumen height, Haugh unit, thick albumen fraction) due to dietary treatments. Also, the tibia strength, Ca content in the tibia ash and bone mineral content, were significantly increased by the dietary treatments. Significant increases in the serum levels of E2, LH, FSH, T3, T4, and GH, and the activity of antioxidant enzymes; SOD, CAT, GSH while reducing the level of MDA, was notable with the treatments. Additionally, reproductive-related genes: ESR1, FSHR, PRLR, GNRH1 were significantly upregulated by the supplementation of GEN and GLY. The efficacy of GEN in relation to the evaluated parameters was superior to that of GLY. Conclusively, we speculate that the improvement on laying performance, egg quality and tibia quality may be related to promoting effect of isoflavones on calcium metabolism, antioxidant function, reproductive hormones and related genes. Therefore, supplemental GEN at a dosage level of 50 mg/kg, can be used to promote laying performance, sustain egg production and maintain the physiological function of young laying hens.