■已发现幽门螺杆菌与牙周炎有关,口腔被认为是幽门螺杆菌胃炎感染的储库。因此,这项研究评估了含有聚赖氨酸和单月桂酸甘油酯的漱口水对抑制幽门螺杆菌生长的作用,生物膜的形成,细胞毒性,粘附能力,cagAmRNA表达,和幽门螺杆菌刺激的促炎细胞因子。
■从口腔中分离出19株幽门螺杆菌菌株。检查了含聚1-赖氨酸和单月桂酸甘油酯的漱口水抑制幽门螺杆菌生长和生物膜形成的能力,并测试了口腔上皮细胞(H357)中的细胞活力,胃腺癌细胞(AGS),和牙周膜细胞(PDL)。此外,测试漱口水降低cagAmRNA表达,对H357和AGS细胞的粘附能力,和在AGS和PDL细胞中用幽门螺杆菌刺激的促炎细胞因子。
■含聚赖氨酸和单月桂酸甘油酯的漱口水在5分钟孵育后可将生物膜根除14.9-19.9%,AGS的细胞活力显示77.2、79.8和100.0%,H357和PDL细胞,分别。此外,含聚赖氨酸和单月桂酸甘油酯的漱口水可以下调cagAmRNA的表达,H357细胞减少幽门螺杆菌粘附约9.5-47.8%,AGS细胞减少24.5-62.9%,减少促炎细胞因子,特别是白细胞介素-8,用幽门螺杆菌刺激。
■含有聚赖氨酸和单月桂酸甘油酯的漱口水可以抑制幽门螺杆菌的生长并降低其毒力表达。漱口水还显示出对口腔和胃细胞的低细胞毒性。
UNASSIGNED: Helicobacter pylori has been found to be related to periodontitis, and the oral cavity has been considered a reservoir for H. pylori gastritis infection. Thus, this study evaluated the effect of mouthwash containing poly l-Lysine and glycerol monolaurate on inhibiting H. pylori growth, biofilm formation, cell cytotoxicity, adhesion ability, cagA mRNA expression, and pro-inflammatory cytokines stimulated by H. pylori.
UNASSIGNED: Nineteen H. pylori strains were isolated from the oral cavity. The effectiveness of mouthwash containing poly l-Lysine and glycerol monolaurate was examined for its ability to inhibit H. pylori growth and biofilm formation and was tested for cell viability in oral epithelial cells (H357), gastric adenocarcinoma cells (AGS), and periodontal ligament cells (PDL). Additionally, the mouthwash was tested for reducing cagA mRNA expression, adhesion ability to H357 and AGS cells, and pro-inflammatory cytokines stimulated with H. pylori in AGS and PDL cells.
UNASSIGNED: The mouthwash containing poly l-Lysine and glycerol monolaurate could eradicate the biofilm by 14.9-19.9% after incubation at 5 min, and cell viability revealed 77.2, 79.8, and 100.0% for AGS, H357, and PDL cells, respectively. Moreover, the mouthwash containing poly l-Lysine and glycerol monolaurate could down-regulate cagA mRNA expression, reduce adhesion of H. pylori by approximately 9.5-47.8% for H357 cells and 24.5-62.9% for AGS cells, and decrease pro-inflammatory cytokines, especially interleukin-8, stimulated with H. pylori.
UNASSIGNED: Mouthwash containing poly l-Lysine and glycerol monolaurate could inhibit H. pylori growth and reduce their virulence expression. The mouthwash also revealed low cytotoxicity to oral and gastric cells.