关键词: ChIP ChIP-seq Chromatin immunoprecipitation Gene Expression RNA-seq Sequencing

Mesh : Software High-Throughput Nucleotide Sequencing / methods Genomics / methods Sequence Analysis, DNA / methods Humans Computational Biology / methods Reproducibility of Results

来  源:   DOI:10.1016/j.xpro.2024.102960   PDF(Pubmed)

Abstract:
The widespread usage of next-generation sequencing methods for functional genomics studies requires standardized tools for consistent visualization of the associated data. Here, we present seqNdisplayR, an R package for plotting standard sequencing data coverage within a genomic region of interest in a customizable and reproducible manner. We describe steps for installing software, preparing data files, choosing options, and plotting data. This tool is readily available for users with no prior experience with R through the \"Shiny app\" interface. For complete details on the use and execution of this protocol, please refer to Lykke-Andersen et al.,1 Gockert et al.,2 and Rouviere et al.3.
摘要:
用于功能基因组学研究的下一代测序方法的广泛使用需要用于相关数据的一致可视化的标准化工具。这里,我们介绍seqNdisplayR,用于以可定制和可重复的方式在感兴趣的基因组区域内绘制标准测序数据覆盖的R包。我们描述了安装软件的步骤,准备数据文件,选择选项,并绘制数据。此工具是随时可用的用户与R通过\"闪亮的应用程序\"界面之前没有经验。有关此协议的使用和执行的完整详细信息,请参阅Lykke-Andersen等人。,1Gockert等人。,2和Rouviere等3.
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