{Reference Type}: Journal Article {Title}: Protocol for generating customizable and reproducible plots of sequencing coverage data using the seqNdisplayR package. {Author}: Lykke-Andersen S;Rouvière JO;Schmid M;Gockert M;Jensen TH; {Journal}: STAR Protoc {Volume}: 5 {Issue}: 2 {Year}: 2024 Jun 21 暂无{DOI}: 10.1016/j.xpro.2024.102960 {Abstract}: The widespread usage of next-generation sequencing methods for functional genomics studies requires standardized tools for consistent visualization of the associated data. Here, we present seqNdisplayR, an R package for plotting standard sequencing data coverage within a genomic region of interest in a customizable and reproducible manner. We describe steps for installing software, preparing data files, choosing options, and plotting data. This tool is readily available for users with no prior experience with R through the "Shiny app" interface. For complete details on the use and execution of this protocol, please refer to Lykke-Andersen et al.,1 Gockert et al.,2 and Rouviere et al.3.