%0 Journal Article %T Protocol for generating customizable and reproducible plots of sequencing coverage data using the seqNdisplayR package. %A Lykke-Andersen S %A Rouvière JO %A Schmid M %A Gockert M %A Jensen TH %J STAR Protoc %V 5 %N 2 %D 2024 Jun 21 %M 38502686 暂无%R 10.1016/j.xpro.2024.102960 %X The widespread usage of next-generation sequencing methods for functional genomics studies requires standardized tools for consistent visualization of the associated data. Here, we present seqNdisplayR, an R package for plotting standard sequencing data coverage within a genomic region of interest in a customizable and reproducible manner. We describe steps for installing software, preparing data files, choosing options, and plotting data. This tool is readily available for users with no prior experience with R through the "Shiny app" interface. For complete details on the use and execution of this protocol, please refer to Lykke-Andersen et al.,1 Gockert et al.,2 and Rouviere et al.3.