PDF(Pubmed)
Abstract：
Background: Fingerprint drug concentrations can be used as a noninvasive and convenient alternative to evaluate adherence to pharmacotherapy. Methods: Fingerprints were applied over glass slides, extracted and analyzed by ultra-high performance LC-MS/MS. The assay and drug adherence questionnaires were applied to 30 epilepsy patients. Results: The assay had linearity in the range 0.05-10 ng fingerprint-1, with precision of 2.16-7.9% and accuracy of 95.0-102.8%. Carbamazepine (CBZ) levels in fingerprints were stable at 45°C for 15 days. Concentrations in patient samples were 0.06-9.28 ng fingerprint-1. A significant difference (p = 0.003) was found between CBZ concentrations in fingerprints between patient groups divided as low and medium/high adherence. Conclusion: This method can potentially be applied to the identification of epilepsy patients with low adherence to CBZ pharmacotherapy.
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摘要：
背景：指纹药物浓度可以用作评估药物治疗依从性的非侵入性且方便的替代方法。方法：在载玻片上应用指纹图谱，通过超高性能LC-MS进行提取和分析。对30例癫痫患者进行了测定和药物依从性问卷。结果：该方法在0.05-10ng指纹图谱1范围内线性关系良好，精密度为2.16-7.9％，准确度为95.0-102.8％。指纹中的卡马西平(CBZ)水平在45°C稳定15天。患者样品中的浓度为0.06-9.28ng指纹-1。在分为低和中/高依从性的患者组之间的指纹中的CBZ浓度之间发现了显着差异（p=0.003）。结论：该方法可用于识别CBZ药物治疗依从性低的癫痫患者。
