%0 Journal Article
%T Determination of carbamazepine in fingerprints: a feasibility study to evaluate adherence in epilepsy patients.
%A Müller IR
%A Grandi VT
%A Santos Paulino SG
%A Villa B
%A Zembruski BM
%A Forcelini CM
%A Hahn SR
%A Antunes MV
%A Linden R
%J Bioanalysis
%V 16
%N 9
%D 2024 Mar 18
%M 38497729
%F 2.695
%R 10.4155/bio-2023-0265
%X Background: Fingerprint drug concentrations can be used as a noninvasive and convenient alternative to evaluate adherence to pharmacotherapy. Methods: Fingerprints were applied over glass slides, extracted and analyzed by ultra-high performance LC-MS/MS. The assay and drug adherence questionnaires were applied to 30 epilepsy patients. Results: The assay had linearity in the range 0.05-10 ng fingerprint-1, with precision of 2.16-7.9% and accuracy of 95.0-102.8%. Carbamazepine (CBZ) levels in fingerprints were stable at 45°C for 15 days. Concentrations in patient samples were 0.06-9.28 ng fingerprint-1. A significant difference (p = 0.003) was found between CBZ concentrations in fingerprints between patient groups divided as low and medium/high adherence. Conclusion: This method can potentially be applied to the identification of epilepsy patients with low adherence to CBZ pharmacotherapy.<BR>[Box: see text].