结晶紫(CV)染色技术代表了开发潜在指纹的一种普遍方法,特别是在胶带表面。然而,该技术需要复杂的配方来增强其性能。在这次调查中,开发了优化的CV染色方案,特征在于在目标基底上不存在残留染料并且在环境光条件下促进指纹可视化的能力。四个捐助者,包括两名男性和两名女性,在各种基材上沉积天然指纹,包括玻璃显微镜载玻片,铝箔,和115克光泽涂布纸,没有任何具体的指导方针。使用氰基丙烯酸酯发烟形成的指纹作为基准,并与通过替代方法产生的指纹进行了对比:CV,Ardrox,罗丹明6G,粉化,和优化的CV染色方案。指纹显影实验以七个不同的时间间隔进行复制,包括1天,1周,1、3、6、9和12个月,产生420个指纹的数据集。指纹识别能力的评估采用了内政部应用科学与技术中心建立的评分系统。结果表明,优化的CV染色技术表现出优异的性能,与其他采用的方法相比,可识别指纹的开发率为92.9%。因此,这种优化的CV染色方法被推荐为一种有效的,快速,和简单的关键染色方法,适用于法医调查中的各种底物。
The crystal violet (CV) staining technique represents a prevalent approach for the development of latent fingerprints, especially on adhesive tape surfaces. Nevertheless, the technique necessitates intricate formulations to augment its performance. In this investigation, an optimized CV staining protocol was developed, characterized by the absence of residual dye on the target substrates and the capability of facilitating fingerprint visualization under ambient light conditions. Four donors, comprising two males and two females, deposited natural fingerprints on various substrates, including glass microscope slides, aluminum foil, and 115 g glossy coated paper, without any specific guidelines. Fingerprints developed using cyanoacrylate fuming served as benchmarks and were contrasted with those generated through alternative methods: CV, ardrox, rhodamine 6G, powdering, and the optimized CV staining protocol. The fingerprint development experiment was replicated at seven distinct time intervals, encompassing 1 day, 1 week, 1, 3, 6, 9, and 12 months, resulting in a dataset of 420 fingerprints. The evaluation of fingerprint identifiability employed a scoring system established by the Home Office Centre for Applied Science and Technology. The results indicated that the optimized CV staining technique demonstrated superior performance, boasting a 92.9% rate of identifiable fingerprint development in contrast to other employed methodologies. Consequently, this optimized CV staining approach is recommended as an efficient, rapid, and straightforward critical dyeing method, applicable to a wide array of substrates in forensic investigations.