Abstract：
The present study was designed to evaluate whether AuNPs (gold nanoparticles) synthesized with the Cynara scolymus (CS) leaf exert protective and/or alleviative effects on arsenic (As)-induced hippocampal neurotoxicity in mice. Neurotoxicity in mice was developed by orally treating 10 mg/kg/day sodium arsenite (NaAsO2) for 21 days. 10 µg/g AuNPs, 1.6 g/kg CS, and 10 µg/g CS-AuNPs were administered orally simultaneously with 10 mg/kg As. CS and CS-AuNPs treatments showed down-regulation of TNF-α and IL-1β levels. CS and CS-AuNPs also ameliorated apoptosis and reduced the alterations in the expression levels of D1 and D2 dopamine receptors induced by As. Simultaneous treatment with CS and CS-AuNPs improved As-induced learning, memory deficits, and motor coordination in mice assessed by water maze and locomotor tests, respectively. The results of this study provide evidence that CS-AuNPs demonstrated neuroprotective roles with antioxidant, anti-inflammatory, and anti-apoptotic effects, as well as improving D1 and D2 signaling, and eventually reversed neurobehavioral impairments.
摘要：
本研究旨在评估与Cynarascolymus（CS）叶合成的AuNPs（金纳米颗粒）是否对砷（As）诱导的小鼠海马神经毒性发挥保护和/或缓解作用。通过口服处理10mg/kg/天的亚砷酸钠(NaAsO2)21天来发展小鼠的神经毒性。10µg/gAuNPs，1.6g/kgCS，和10µg/gCS-AuNP与10mg/kgAs同时口服给药。CS和CS-AuNP治疗显示TNF-α和IL-1β水平下调。CS和CS-AuNP还改善了细胞凋亡，并减少了As诱导的D1和D2多巴胺受体表达水平的改变。CS和CS-AuNP同时治疗可改善As诱导学习，记忆缺陷,通过水迷宫和运动测试评估小鼠的运动协调性，分别。这项研究的结果提供了证据，CS-AuNPs证明了抗氧化剂的神经保护作用，抗炎,和抗凋亡作用，以及改进D1和D2信令，最终逆转了神经行为障碍。
