Abstract：
The high-purity phycocyanin has a high commercial value. Most current purification methods of C-phycocyanin involve multiple steps, which are complicated and time-consuming. To solve the problem, this research was studied, and an efficient affinity chromatography purification for C-phycocyanin from Spirulina platensis was developed. Through molecular docking simulation, virtual screening of ligands was performed, and ursolic acid was identified as the specific affinity ligand, which coupled to Affi-Gel 102 gel via 1-ethyl (3-dimethylaminopropyl)-3-carbodiimide, hydrochloride as coupling agent. With this customized and synthesized resin, a high-efficiency one-step purification procedure for C-phycocyanin was developed and optimized, the purity was determined to be 4.53, and the yield was 69 %. This one-step purification protocol provides a new approach for purifying other phycobilin proteins.
摘要：
高纯度的藻蓝蛋白具有很高的商业价值。目前大多数C-藻蓝蛋白的纯化方法涉及多个步骤，这是复杂和耗时的。为了解决问题,这项研究进行了研究，并开发了一种有效的亲和层析纯化螺旋藻中的C-藻蓝蛋白。通过分子对接模拟,进行了配体的虚拟筛选，熊果酸被鉴定为特异性亲和配体，通过1-乙基（3-二甲基氨基丙基）-3-碳二亚胺与Affi-Gel102凝胶偶联，盐酸盐作为偶联剂。有了这种定制和合成树脂，开发并优化了C-藻蓝蛋白的高效一步纯化程序，纯度测定为4.53,收率为69%。这种一步纯化方案为纯化其他藻胆素蛋白提供了一种新的方法。
