{Reference Type}: Journal Article
{Title}: A rapid one-step affinity purification of C-phycocyanin from Spirulina platensis.
{Author}: Shi K;Wang W;Sun J;Jiang C;Hao J;
{Journal}: J Chromatogr A
{Volume}: 1720
{Issue}: 0
{Year}: 2024 Apr 12
{Factor}: 4.601
{DOI}: 10.1016/j.chroma.2024.464801
{Abstract}: The high-purity phycocyanin has a high commercial value. Most current purification methods of C-phycocyanin involve multiple steps, which are complicated and time-consuming. To solve the problem, this research was studied, and an efficient affinity chromatography purification for C-phycocyanin from Spirulina platensis was developed. Through molecular docking simulation, virtual screening of ligands was performed, and ursolic acid was identified as the specific affinity ligand, which coupled to Affi-Gel 102 gel via 1-ethyl (3-dimethylaminopropyl)-3-carbodiimide, hydrochloride as coupling agent. With this customized and synthesized resin, a high-efficiency one-step purification procedure for C-phycocyanin was developed and optimized, the purity was determined to be 4.53, and the yield was 69 %. This one-step purification protocol provides a new approach for purifying other phycobilin proteins.