%0 Journal Article
%T A rapid one-step affinity purification of C-phycocyanin from Spirulina platensis.
%A Shi K
%A Wang W
%A Sun J
%A Jiang C
%A Hao J
%J J Chromatogr A
%V 1720
%N 0
%D 2024 Apr 12
%M 38479154
%F 4.601
%R 10.1016/j.chroma.2024.464801
%X The high-purity phycocyanin has a high commercial value. Most current purification methods of C-phycocyanin involve multiple steps, which are complicated and time-consuming. To solve the problem, this research was studied, and an efficient affinity chromatography purification for C-phycocyanin from Spirulina platensis was developed. Through molecular docking simulation, virtual screening of ligands was performed, and ursolic acid was identified as the specific affinity ligand, which coupled to Affi-Gel 102 gel via 1-ethyl (3-dimethylaminopropyl)-3-carbodiimide, hydrochloride as coupling agent. With this customized and synthesized resin, a high-efficiency one-step purification procedure for C-phycocyanin was developed and optimized, the purity was determined to be 4.53, and the yield was 69 %. This one-step purification protocol provides a new approach for purifying other phycobilin proteins.