背景:甜菜滤饼(BFC)是制糖工业产生的食品级固体废物,构成永久污染源。蓝细菌被认为是各种生物活性化合物如藻蓝蛋白色素的可持续资源,具有有价值的应用。这项研究旨在使用甜菜滤饼提取物(BFCE)作为替代培养基,用于经济地培养蓝细菌Leptolyngbyasp。SSI24PP723083,然后生物精制生物活性成分,如藻蓝蛋白色素,可用于生产硒纳米颗粒。
结果:分批实验的结果表明,蛋白质含量最高的是BG11培养基(47.9%);但是,最大碳水化合物和脂质含量在25%BFCE(15.25%和10.23%,分别)。此外,75%BFCE培养基刺激了藻蓝蛋白含量(25.29mg/g),与BG11(22.8mg/g)相比变化不明显。此外,在BG11和75%BFCE上培养的LeptolyngbyaspSSI24的粗藻蓝蛋白提取物成功地产生了球形硒纳米颗粒(Se-NP),两种提取物的平均尺寸分别为95和96nm,分别。此外,XRD结果表明,生物合成的Se-NP具有结晶性质。此外,在对照和75%BFCE处理中,生物合成Se-NP的Zeta电位等于-17mV和-15.03mV,分别,表明其稳定性。生物合成的Se-NP对革兰氏阳性细菌的效力高于革兰氏阴性细菌。此外,与75%BFCE培养基相比,来自BG11的生物合成Se-NP具有更高的抗氧化活性,IC50为60±0.7。Further,从75%BFCE上培养的Leptolynbyasp提取的藻蓝蛋白生物合成的Se-NP对人乳腺癌细胞系具有很强的抗癌活性,IC50为17.31±0.63µg/ml。
结论:补充BFCE的培养基可用于藻蓝蛋白积累的蓝细菌菌株的培养,用于具有生物学应用的硒纳米颗粒的绿色合成。
BACKGROUND: Beet filter cake (BFC) is a food-grade solid waste produced by the sugar industry, constituting a permanent source of pollution. Cyanobacteria are considered a sustainable resource for various bioactive compounds such as
phycocyanin pigment with valuable applications. This study aimed to use beet filter cake extract (BFCE) as an alternative medium for the economic cultivation of cyanobacterium Leptolyngbya sp. SSI24 PP723083, then biorefined the bioactive component such as
phycocyanin pigment that could be used in the production of selenium nanoparticles.
RESULTS: The results of the batch experiment displayed that the highest protein content was in BG11medium (47.9%); however, the maximum carbohydrate and lipid content were in 25% BFCE (15.25 and 10.23%, respectively). In addition, 75% BFCE medium stimulated the
phycocyanin content (25.29 mg/g) with an insignificant variation compared to BG11 (22.8 mg/g). Moreover, crude
phycocyanin extract from Leptolyngbya sp SSI24 cultivated on BG11 and 75% BFCE successfully produced spherical-shaped selenium nanoparticles (Se-NPs) with mean sizes of 95 and 96 nm in both extracts, respectively. Moreover, XRD results demonstrated that the biosynthesized Se-NPs have a crystalline nature. In addition, the Zeta potential of the biosynthesized Se-NPs equals - 17 mV and - 15.03 mV in the control and 75% BFCE treatment, respectively, indicating their stability. The biosynthesized Se-NPs exhibited higher effectiveness against Gram-positive bacteria than Gram-negative bacteria. Moreover, the biosynthesized Se-NPs from BG11 had higher antioxidant activity with IC50 of 60 ± 0.7 compared to 75% BFCE medium. Further, Se-NPs biosynthesized from
phycocyanin extracted from Leptolyngbya sp cultivated on 75% BFCE exhibited strong anticancer activity with IC50 of 17.31 ± 0.63 µg/ml against the human breast cancer cell line.
CONCLUSIONS: The BFCE-supplemented medium can be used for the cultivation of cyanobacterial strain for the phycocyanin accumulation that is used for the green synthesis of selenium nanoparticles that have biological applications.