PDF(Pubmed)
Abstract：
The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP. Here we report a high-resolution cryogenic electron microscopy structure of the 20S U5 snRNP, shedding light on the mutually exclusive interfaces utilized during tri-snRNP assembly and the role of the CD2BP2 in facilitating this process.
摘要：
20SU5小核核糖核蛋白颗粒（snRNP）是17亚基RNA-蛋白质复合物，是U4/U6的前体。U5三-snRNP,预催化剪接体的主要组成部分。CD2BP2是20SU5snRNP的标志性蛋白，不存在于成熟的tri-snRNP中。在这里，我们报告了20SU5snRNP的高分辨率低温电子显微镜结构，在tri-snRNP组装期间使用的互斥接口上的发光以及CD2BP2在促进该过程中的作用。
