%0 Journal Article
%T Structure of the human 20S U5 snRNP.
%A Schneider S
%A Brandina I
%A Peter D
%A Lagad S
%A Fraudeau A
%A Portell-Montserrat J
%A Tholen J
%A Zhao J
%A Galej WP
%J Nat Struct Mol Biol
%V 31
%N 5
%D 2024 May 11
%M 38467877
%F 18.361
%R 10.1038/s41594-024-01250-5
%X The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP. Here we report a high-resolution cryogenic electron microscopy structure of the 20S U5 snRNP, shedding light on the mutually exclusive interfaces utilized during tri-snRNP assembly and the role of the CD2BP2 in facilitating this process.