{Reference Type}: Journal Article
{Title}: Structure of the human 20S U5 snRNP.
{Author}: Schneider S;Brandina I;Peter D;Lagad S;Fraudeau A;Portell-Montserrat J;Tholen J;Zhao J;Galej WP;
{Journal}: Nat Struct Mol Biol
{Volume}: 31
{Issue}: 5
{Year}: 2024 May 11
{Factor}: 18.361
{DOI}: 10.1038/s41594-024-01250-5
{Abstract}: The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP. Here we report a high-resolution cryogenic electron microscopy structure of the 20S U5 snRNP, shedding light on the mutually exclusive interfaces utilized during tri-snRNP assembly and the role of the CD2BP2 in facilitating this process.