Abstract:
Tara (Caesalpinia spinosa, Leguminosae) seed germ (TSG), a by-product of tara gum (E417) extraction, has been used as a protein- and polyphenol-rich food ingredient for human and animal nutrition. Nevertheless, TSG is the alleged culprit for a recent foodborne outbreak of even severe acute illnesses that have affected hundreds of individuals in the USA, perhaps triggered by nonprotein amino acids such as baikiain. Herein, the composition of TSG has been characterized at molecular level, with a focus on proteins, phenolics, lipids, and mineral composition. TSG contains 43.4 % (w/w) proteins, tentatively identified for the first time by proteomics, and 14 % lipids, consisting of 83.6 % unsaturated fatty acids, especially linoleic acid. Ash is surprising high (6.5 %) because of an elevated concentration of P, K, Ca, and Mg. The detection of a rare earth element such as gadolinium (Gd, 1.6 mg kg-1), likely sourced from anthropogenic pollution, suggests alternative hypotheses for the origin of TSG hazards.
摘要:
塔拉(刺槐,豆科)种子胚芽(TSG),塔拉胶(E417)提取的副产品,已被用作富含蛋白质和多酚的食品成分,用于人类和动物营养。然而,TSG是最近食源性疾病爆发的罪魁祸首,这些疾病甚至影响了美国数百人,可能是由非蛋白质氨基酸如baikain引发的。在这里,TSG的组成已经在分子水平上进行了表征,专注于蛋白质,酚类物质,脂质,和矿物成分。TSG含有43.4%(w/w)的蛋白质,首次通过蛋白质组学初步鉴定,和14%的脂质,由83.6%的不饱和脂肪酸组成,尤其是亚油酸.灰分是惊人的高(6.5%),因为P的浓度升高,K,Ca,Mg。诸如钆(Gd,1.6mgkg-1),可能来自人为污染,为TSG危险的起源提出了替代假设。