Abstract：
Microneedles are minimally-invasive devices with the unique capability of bypassing physiological barriers. Hence, they are widely used for different applications from drug/vaccine delivery to diagnosis and cosmetic fields. Recently, natural biopolymers (particularly carbohydrates and proteins) have garnered attention as safe and biocompatible materials with tailorable features for microneedle construction. Several review articles have dealt with carbohydrate-based microneedles. This review aims to highlight the less-noticed role of proteins through a systematic search strategy based on the PRISMA guideline from international databases of PubMed, Science Direct, Scopus, and Google Scholar. Original English articles with the keyword \"microneedle(s)\" in their titles along with at least one of the keywords \"biopolymers, silk, gelatin, collagen, zein, keratin, fish-scale, mussel, and suckerin\" were collected and those in which the proteins undertook a structural role were screened. Then, we focused on the structures and applications of protein-based microneedles. Also, the unique features of some protein biopolymers that make them ideal for microneedle construction (e.g., excellent mechanical strength, self-adhesion, and self-assembly), as well as the challenges associated with them were reviewed. Altogether, the proteins identified so far seem not only promising for the fabrication of \"better\" microneedles in the future but also inspiring for designing biomimetic structural biopolymers with ideal characteristics.
摘要：
微针是具有绕过生理屏障的独特能力的微创设备。因此，它们广泛用于从药物/疫苗递送到诊断和化妆品领域的不同应用。最近,天然生物聚合物（特别是碳水化合物和蛋白质）作为具有可定制特征的微针结构的安全和生物相容性材料而受到关注。几篇综述文章涉及基于碳水化合物的微针。这篇综述旨在通过基于PubMed国际数据库的PRISMA指南的系统搜索策略来强调蛋白质的较少注意的作用，科学直接，Scopus,谷歌学者。标题中带有关键字“微针”以及至少一个关键字“生物聚合物”的原始英文文章，丝绸,明胶,胶原蛋白，zein,角蛋白,鱼鳞,贻贝,收集了suckerin\“，并筛选了其中蛋白质具有结构作用的蛋白质。然后，我们专注于基于蛋白质的微针的结构和应用。此外，一些蛋白质生物聚合物的独特特征，使它们成为微针构建的理想选择(例如，优异的机械强度，自粘,和自组装)，以及与之相关的挑战进行了审查。总之,到目前为止鉴定的蛋白质似乎不仅有望在未来制造“更好”的微针，而且对设计具有理想特性的仿生结构生物聚合物也很有启发。
