■病原菌是大多数皮肤病的病因,但是耐药性和环境退化等问题促使人们需要研究替代治疗方法。据报道,蚕茧提取物具有抗氧化性能。在丝绸加工过程中,蚕茧的脱胶会产生含有天然活性物质的副产品。发现这些物质对细菌生长有抑制作用,DNA合成,溶血的发病机制,和生物膜的形成。因此,蚕茧提取物可用于预防和治疗皮肤致病菌感染的治疗应用。
■通过用蒸馏水煮沸9小时和12小时,获得了有p的丝茧(SCP)和无p的丝茧(SCWP)的提取物,并与煮1小时的蚕蛹(SP)提取物进行比较。提取物中的活性化合物,包括没食子酸和槲皮素,使用高效液相色谱法(HPLC)测定。此外,用Folin-Ciocalteu法和氯化铝比色法研究了提取物中的总酚和黄酮含量,分别。为了评估抗氧化活性,使用2,2-二苯基-1-吡啶酰肼(DPPH)自由基清除测定法评估提取物。此外,确定了蚕丝提取物和植物化学化合物对皮肤致病菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。这项研究评估了提取物和植物化学化合物对生长抑制的影响,生物膜的形成,溶血保护,和细菌的DNA合成。
■蚕丝提取物的HPLC表征显示没食子酸含量最高,特别是在SCP(8.638-31.605mg/g提取物)和SP(64.530mg/g提取物)中;而槲皮素化合物仅在SCWP(0.021-0.031mg/g提取物)中检测到。蚕丝提取物中的总酚和类黄酮具有抗氧化和抗菌活性。此外,SCP在9小时和12小时显示出最高的抗菌活性,对包括金黄色葡萄球菌在内的皮肤致病菌的最低MIC和MBC为50-100mg/mL,耐甲氧西林金黄色葡萄球菌(MRSA),痤疮杆菌和铜绿假单胞菌。因此,SCP提取物和含有没食子酸和槲皮素的非丝胶化合物对皮肤致病菌的生长和DNA合成均表现出最强的抑制作用。抑制细菌发病机制,包括预制和成熟的生物膜,和溶血活性,在SCP提取物和非丝胶化合物中也被发现。结果表明,丝加工的副产品可以作为天然酚类和类黄酮抗氧化剂的替代来源,可用于预防和治疗致病性细菌皮肤感染的治疗应用。
UNASSIGNED: Pathogenic bacteria are the cause of most skin diseases, but issues such as resistance and environmental degradation drive the need to research alternative treatments. It is reported that
silk cocoon extract possesses antioxidant properties. During
silk processing, the degumming of
silk cocoons creates a byproduct that contains natural active substances. These substances were found to have inhibitory effects on bacterial growth, DNA synthesis, the pathogenesis of hemolysis, and biofilm formation. Thus,
silk cocoon extracts can be used in therapeutic applications for the prevention and treatment of skin pathogenic bacterial infections.
UNASSIGNED: The extract of
silk cocoons with pupae (SCP) and
silk cocoons without pupae (SCWP) were obtained by boiling with distilled water for 9 h and 12 h, and were compared to silkworm pupae (SP) extract that was boiled for 1 h. The active compounds in the extracts, including gallic acid and quercetin, were determined using high-performance liquid chromatography (HPLC). Furthermore, the total phenolic and flavonoid content in the extracts were investigated using the Folin-Ciocalteu method and the aluminum chloride colorimetric method, respectively. To assess antioxidant activity, the extracts were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Additionally, the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of silk extracts and phytochemical compounds were determined against skin pathogenic bacteria. This study assessed the effects of the extracts and phytochemical compounds on growth inhibition, biofilm formation, hemolysis protection, and DNA synthesis of bacteria.
UNASSIGNED: The HPLC characterization of the
silk extracts showed gallic acid levels to be the highest, especially in SCP (8.638-31.605 mg/g extract) and SP (64.530 mg/g extract); whereas quercetin compound was only detected in SCWP (0.021-0.031 mg/g extract). The total phenolics and flavonoids in silk extracts exhibited antioxidant and antimicrobial activity. Additionally, SCP at 9 h and 12 h revealed the highest anti-bacterial activity, with the lowest MIC and MBC of 50-100 mg/mL against skin pathogenic bacteria including Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Cutibacterium acnes and Pseudomonas aeruginosa. Hence, SCP extract and non-sericin compounds containing gallic acid and quercetin exhibited the strongest inhibition of both growth and DNA synthesis on skin pathogenic bacteria. The suppression of bacterial pathogenesis, including preformed and matured biofilms, and hemolysis activity, were also revealed in SCP extract and non-sericin compounds. The results show that the byproduct of silk processing can serve as an alternative source of natural phenolic and flavonoid antioxidants that can be used in therapeutic applications for the prevention and treatment of pathogenic bacterial skin infections.