PDF(Pubmed)
Abstract：
Desulfofundulus kuznetsovii is a thermophilic, spore-forming sulphate-reducing bacterium in the family Peptococcaceae. In this study, we describe a newly isolated strain of D. kuznetsovii, strain TPOSR, and compare its metabolism to the type strain D. kuznetsovii 17T. Both strains grow on a large variety of alcohols, such as methanol, ethanol and propane-diols, coupled to the reduction of sulphate. Strain 17T metabolizes methanol via two routes, one involving a cobalt-dependent methyl transferase and the other using a cobalt-independent alcohol dehydrogenase. However, strain TPOSR, which shares 97% average nucleotide identity with D. kuznetsovii strain 17T, lacks several genes from the methyl transferase operon found in strain 17T. The gene encoding the catalytically active methyl transferase subunit B is missing, indicating that strain TPOSR utilizes the alcohol dehydrogenase pathway exclusively. Both strains grew with methanol during cobalt starvation, but growth was impaired. Strain 17T was more sensitive to cobalt deficiency, due to the repression of its methyl transferase system. Our findings shed light on the metabolic diversity of D. kuznetsovii and their metabolic differences of encoding one or two routes for the conversion of methanol.
摘要：
Desulfofunduluskuznetsovii是嗜热的，Peptococaceae家族中形成孢子的硫酸盐还原细菌。在这项研究中,我们描述了一种新分离的D.kuznetsovii菌株，菌株TPOSR，并将其代谢与D.kuznetsovii17T型菌株进行比较。两种菌株都生长在各种各样的醇上，如甲醇，乙醇和丙烷二醇，再加上硫酸盐的还原。菌株17T通过两种途径代谢甲醇，一种涉及钴依赖性甲基转移酶，另一种涉及钴依赖性醇脱氢酶。然而,菌株TPOSR，与D.kuznetsovii菌株17T共有97%的平均核苷酸同一性，缺乏来自菌株17T中发现的甲基转移酶操纵子的几个基因。编码具有催化活性的甲基转移酶亚基B的基因缺失，表明菌株TPOSR仅利用醇脱氢酶途径。两种菌株在钴饥饿期间都与甲醇一起生长，但增长受损。菌株17T对钴缺乏更敏感，由于其甲基转移酶系统的抑制。我们的发现揭示了D.kuznetsovii的代谢多样性及其编码一种或两种甲醇转化途径的代谢差异。
