一部小说,孢子形成,嗜酸和耐金属硫酸盐还原细菌,菌株OLT,是从金矿开采现场尾矿坝的微生物垫中分离出来的。细胞是略微弯曲的不运动棒,直径0.5µm,长度2.0-3.0µm。细胞染色为革兰氏阴性,尽管超薄层的电子显微镜显示了革兰氏阳性细胞结构。OLT在pH4.0-7.0下生长,最适温度为5.5。OLT利用H2,乳酸,丙酮酸,苹果酸,甲酸盐,丙酸盐,乙醇,甘油,葡萄糖,果糖,蔗糖,蛋白胨和胰胨作为硫酸盐还原的电子供体。硫酸盐,亚硫酸盐,硫代硫酸盐,在乳酸存在下,硝酸盐和富马酸盐被用作电子受体。元素硫,铁(III),和砷酸盐不作为电子受体。主要的细胞脂肪酸是C16:1ω7c(39.0%)和C16:0(12.1%)。OLT的基因组草图大小为5.29Mb,包含4909个蛋白质编码基因。16SrRNA基因序列将OLT置于厚壁门中,类梭菌,Peptococaceae,脱硫磷属。Desulfosporosinus硝基还原酶59.4BT是最接近的亲戚,序列相似性为97.6%。根据表型和系统发育特征,菌株OLT代表了Desulfosporosinus属中的一个新物种,为此,我们建议将其命名为Desulfosporosinusmetalliduranssp。11月。与OLT型菌株(=DSM104464T=VKM-3021T)。
A novel, spore-forming, acidophilic and metal-resistant sulfate-reducing bacterium, strain OLT, was isolated from a microbial mat in a tailing dam at a gold ore mining site. Cells were slightly curved immotile rods, 0.5 µm in diameter and 2.0-3.0 µm long. Cells were stained Gram-negative, despite the Gram-positive cell structure revealed by electron microscopy of ultrathin layers. OLT grew at pH 4.0-7.0 with an optimum at 5.5. OLT utilised H2, lactate, pyruvate, malate, formate, propionate, ethanol, glycerol, glucose, fructose, sucrose, peptone and tryptone as electron donors for sulfate reduction. Sulfate, sulfite, thiosulfate, nitrate and fumarate were used as electron acceptors in the presence of lactate. Elemental sulfur, iron (III), and arsenate did not serve as electron acceptors. The major cellular fatty acids were C16:1ω7c (39.0 %) and C16 : 0 (12.1 %). The draft genome of OLT was 5.29 Mb in size and contained 4909 protein-coding genes. The 16S rRNA gene sequence placed OLT within the phylum Firmicutes, class Clostridia, family
Peptococcaceae, genus Desulfosporosinus. Desulfosporosinus nitroreducens 59.4BT was the closest relative with 97.6 % sequence similarity. On the basis of phenotypic and phylogenetic characteristics, strain OLT represents a novel species within the genus Desulfosporosinus, for which we propose the name Desulfosporosinus metallidurans sp. nov. with the type strain OLT (=DSM 104464T=VKM В-3021T).