台湾每年生产大量的肉鸡肝脏,但一直被认为是废物。我们的团队已成功申请了具有多种生物功能的鸡肝水解物(CLH)的专利并对其进行了表征。长期饮酒会导致肝骨病甚至肝炎,肝硬化,和癌症。本研究旨在研究基于CLH的补充剂(GBHP01™)对慢性饮酒的肝保护作用。结果表明,GBHP01™可以减少(p<0.05)增大的肝脏大小,脂质积累/脂肪变性评分,和更高的血清AST,ALT,γ-GT,甘油三酯,和酒精液体饮食引起的胆固醇水平。GBHP01™通过降低TBARS减少酒精性液体饮食喂养小鼠的肝脏炎症和细胞凋亡,白细胞介素-6,白细胞介素-1β,和肿瘤坏死因子-α水平,增加降低的GSH/TEAC水平和SOD活性,CAT和GPx,以及下调CYP2E1,BAX/BCL2,裂解CASPASE-9/总CASPASE-9和活性CASPASE-3/Pro-CASPASE-3(p<0.05)。此外,GBHP01™肝脏酒精代谢升高(ADH和ALDH活性)(p<0.05)。总之,这项研究证明了GBHP01™对饮酒的肝脏保护作用。
Tons of broiler livers are produced yearly in Taiwan but always considered waste. Our team has successfully patented and characterized a chicken-liver hydrolysate (CLH) with several biofunctions. Chronic alcohol consumption causes hepatosteatosis or even hepatitis, cirrhosis, and cancers. This study was to investigate the hepatoprotection of CLH-based supplement (GBHP01™) against chronic alcohol consumption. Results showed that GBHP01™ could reduce (p < .05) enlarged liver size, lipid accumulation/steatosis scores, and higher serum AST, ALT, γ-GT, triglyceride, and cholesterol levels induced by an alcoholic liquid diet. GBHP01™ reduced liver inflammation and apoptosis in alcoholic liquid-diet-fed mice via decreasing TBARS, interleukin-6, interleukin-1β, and tumor necrosis factor-α levels, increasing reduced GSH/TEAC levels and activities of SOD, CAT and GPx, as well as downregulating CYP2E1, BAX/BCL2, Cleaved CASPASE-9/Total CASPASE-9 and Active CASPASE-3/Pro-CASPASE-3 (p < .05). Furthermore, GBHP01™ elevated hepatic alcohol metabolism (ADH and ALDH activities) (p < .05). In conclusion, this study prove the hepatoprotection of GBHP01™ against alcohol consumption.