PDF(Pubmed)
Abstract：
In this study, we devised a nanogold lateral flow immunoassay (LFA-CPV antigen test) for detecting canine parvovirus (CPV) in living attenuated CPV vaccines. We conducted instrumental characterization of the prepared nanogold particles and the developed LFA-CPV antigen test was rigorously evaluated for its performance verification including limit of detection, sensitivity, specificity, selectivity and accuracy. The LFA-CPV antigen test demonstrated strong performance when assessed against qPCR using different batches of live attenuated CPV vaccines, indicated a sensitivity of 96.4%, specificity of 88.2%, and an overall accuracy of 95%. These results suggest that the developed LFA-CPV antigen test could serve as a viable alternative for evaluation live attenuated CPV vaccines, and provide it as a point of care test for CPV diagnosis, offering a potential substitute for traditional laboratory methods, particularly qPCR.
摘要：
在这项研究中,我们设计了一种纳米金侧流免疫测定法(LFA-CPV抗原试验),用于检测犬细小病毒(CPV)在CPV减毒活疫苗中.我们对制备的纳米金颗粒进行了仪器表征，并严格评估了开发的LFA-CPV抗原测试的性能验证，包括检测限，灵敏度,特异性，选择性和准确性。当使用不同批次的减毒活疫苗对qPCR进行评估时，LFA-CPV抗原测试显示出强大的性能。灵敏度为96.4%，特异性88.2%，总体准确率为95%。这些结果表明，开发的LFA-CPV抗原测试可以作为评估减毒活疫苗的可行替代方法。并提供它作为CPV诊断的护理点测试，为传统的实验室方法提供了潜在的替代品，特别是qPCR。
