{Reference Type}: Journal Article
{Title}: Sensitivity of lateral flow technique for diagnosis of canine parvovirus.
{Author}: Abousenna MS;Sayed RH;E SA;Shasha FA;El Sawy SEA;Darwish DM;
{Journal}: Sci Rep
{Volume}: 14
{Issue}: 1
{Year}: 2024 03 1
{Factor}: 4.996
{DOI}: 10.1038/s41598-024-55548-x
{Abstract}: In this study, we devised a nanogold lateral flow immunoassay (LFA-CPV antigen test) for detecting canine parvovirus (CPV) in living attenuated CPV vaccines. We conducted instrumental characterization of the prepared nanogold particles and the developed LFA-CPV antigen test was rigorously evaluated for its performance verification including limit of detection, sensitivity, specificity, selectivity and accuracy. The LFA-CPV antigen test demonstrated strong performance when assessed against qPCR using different batches of live attenuated CPV vaccines, indicated a sensitivity of 96.4%, specificity of 88.2%, and an overall accuracy of 95%. These results suggest that the developed LFA-CPV antigen test could serve as a viable alternative for evaluation live attenuated CPV vaccines, and provide it as a point of care test for CPV diagnosis, offering a potential substitute for traditional laboratory methods, particularly qPCR.