%0 Journal Article
%T Sensitivity of lateral flow technique for diagnosis of canine parvovirus.
%A Abousenna MS
%A Sayed RH
%A E SA
%A Shasha FA
%A El Sawy SEA
%A Darwish DM
%J Sci Rep
%V 14
%N 1
%D 2024 03 1
%M 38424259
%F 4.996
%R 10.1038/s41598-024-55548-x
%X In this study, we devised a nanogold lateral flow immunoassay (LFA-CPV antigen test) for detecting canine parvovirus (CPV) in living attenuated CPV vaccines. We conducted instrumental characterization of the prepared nanogold particles and the developed LFA-CPV antigen test was rigorously evaluated for its performance verification including limit of detection, sensitivity, specificity, selectivity and accuracy. The LFA-CPV antigen test demonstrated strong performance when assessed against qPCR using different batches of live attenuated CPV vaccines, indicated a sensitivity of 96.4%, specificity of 88.2%, and an overall accuracy of 95%. These results suggest that the developed LFA-CPV antigen test could serve as a viable alternative for evaluation live attenuated CPV vaccines, and provide it as a point of care test for CPV diagnosis, offering a potential substitute for traditional laboratory methods, particularly qPCR.