PDF(Pubmed)
Abstract：
Controlled environment agriculture (CEA), or indoor agriculture, encompasses non-traditional farming methods that occur inside climate-controlled structures (e.g., greenhouses, warehouses, high tunnels) allowing for year-round production of fresh produce such as leaf lettuce. However, recent outbreaks and recalls associated with hydroponically grown lettuce contaminated with human pathogens have raised concerns. Few studies exist on the food safety risks during hydroponic cultivation of leaf lettuce; thus, it is important to identify contributing risk factors and potential mitigation strategies to prevent foodborne transmission via hydroponically grown produce. In this study, the concentration of infectious Tulane virus (TV), a human norovirus surrogate, in hydroponic nutrient solution at 15 °C, 25 °C, 30 °C, and 37 °C was determined over a duration of 21 days to mimic the time from seedling to mature lettuce. The mean log PFU reduction for TV was 0.86, 1.80, 2.87, and ≥ 3.77 log10 at 15 °C, 25 °C, 30 °C, and 37 °C, respectively, at the end of the 21-day period. Similarly, average decimal reduction values (D-values) of TV at 15 °C, 25 °C, 30 °C, and 37 °C were 48.0, 11.3, 8.57, and 7.02 days, respectively. This study aids in the (i) identification of possible food safety risks associated with hydroponic systems specifically related to nutrient solution temperature and (ii) generation of data to perform risk assessments within CEA leaf lettuce operations to inform risk management strategies for the reduction of foodborne outbreaks, fresh produce recalls, and economic losses.
摘要：
受控环境农业（CEA），或者室内农业，包括发生在气候控制结构内部的非传统耕作方法(例如，温室,仓库,高隧道）允许全年生产生菜等新鲜农产品。然而,最近与水培种植的生菜污染人类病原体有关的疫情和召回事件引起了人们的关注。关于叶莴苣水培过程中食品安全风险的研究很少；因此，重要的是要确定起作用的风险因素和潜在的缓解策略，以防止食源性传播通过水培种植的农产品。在这项研究中,传染性杜兰病毒（TV）的浓度，人类诺如病毒替代品,在15°C的水培营养液中，25°C,30°C,在21天的持续时间内测定37°C以模拟从幼苗到成熟莴苣的时间。在15°C下，TV的平均logPFU降低为0.86、1.80、2.87和≥3.77log10，25°C,30°C,37°C,分别,在21天期限结束时。同样，电视在15°C时的平均十进制减少值(D值)，25°C,30°C,37°C分别为48.0、11.3、8.57和7.02天，分别。本研究有助于（i）识别与营养液温度相关的水培系统可能存在的食品安全风险，以及（ii）生成数据以在CEA生菜叶操作中进行风险评估，从而为减少食源性疾病的风险管理策略提供信息爆发，新鲜农产品召回，和经济损失。
