PDF(Pubmed)
Abstract：
UNASSIGNED: Respiratory viral infections are common in febrile infants ≤90 days. However, the detection of viruses other than enterovirus in the blood and cerebrospinal fluid (CSF) of young infants is not well defined. We sought to quantify the occurrence of respiratory viruses in the blood and CSF of febrile infants ≤90 days.
UNASSIGNED: We conducted a nested cohort study examining plasma and CSF samples from febrile infants 15-90 days via rtPCR. The samples were tested for respiratory viruses (respiratory syncytial virus, influenza, enterovirus, parechovirus, adenovirus, bocavirus). Clinical and laboratory data were also collected to determine the presence of serious bacterial infections (SBI).
UNASSIGNED: Twenty-four percent (30 of 126) of infants had plasma/CSF specimens positive for a respiratory virus. Enterovirus and parechovirus were the most commonly detected respiratory viruses. Viral positivity was highest in plasma samples at 25% (27 of 107) compared with CSF samples at 15% (nine of 62). SBIs (specifically urinary tract infections) were less common in infants with a sample positive for a respiratory virus compared to those without a virus detected (3% vs. 26%, p = 0.008).
UNASSIGNED: Our findings support the use of molecular diagnostics to include the identification of parechovirus in addition to enterovirus in febrile infants ≤90 days. Additionally, these data support the utilization of blood specimens to diagnose enterovirus and parechovirus infections in febrile infants ≤90 days.
UNASSIGNED: We conducted a nested cohort study examining plasma and CSF samples from febrile infants 15-90 days via rtPCR. The samples were tested for respiratory viruses (respiratory syncytial virus, influenza, enterovirus, parechovirus, adenovirus, bocavirus). Clinical and laboratory data were also collected to determine the presence of serious bacterial infections (SBI).
UNASSIGNED: Twenty-four percent (30 of 126) of infants had plasma/CSF specimens positive for a respiratory virus. Enterovirus and parechovirus were the most commonly detected respiratory viruses. Viral positivity was highest in plasma samples at 25% (27 of 107) compared with CSF samples at 15% (nine of 62). SBIs (specifically urinary tract infections) were less common in infants with a sample positive for a respiratory virus compared to those without a virus detected (3% vs. 26%, p = 0.008).
UNASSIGNED: Our findings support the use of molecular diagnostics to include the identification of parechovirus in addition to enterovirus in febrile infants ≤90 days. Additionally, these data support the utilization of blood specimens to diagnose enterovirus and parechovirus infections in febrile infants ≤90 days.
摘要：
■呼吸道病毒感染常见于≤90天的发热婴儿。然而,在年轻婴儿的血液和脑脊液(CSF)中除肠道病毒以外的病毒的检测尚不明确.我们试图量化≤90天发热婴儿血液和CSF中呼吸道病毒的发生。
我们进行了一项巢式队列研究，通过rtPCR检查15-90天发热婴儿的血浆和CSF样本。对样品进行了呼吸道病毒（呼吸道合胞病毒，流感,肠病毒,副病毒,腺病毒，博卡病毒）。还收集临床和实验室数据以确定严重细菌感染(SBI)的存在。
■24%（126个婴儿中有30个）的血浆/CSF标本对呼吸道病毒呈阳性。肠道病毒和旁病毒是最常见的呼吸道病毒。血浆样品中的病毒阳性率最高，为25％（107个中的27个），而CSF样品为15％（62个中的9个）。与未检测到病毒的婴儿相比，SBI（特别是尿路感染）在呼吸道病毒样本呈阳性的婴儿中不太常见（3％vs.26%,p=0.008)。
■我们的研究结果支持在≤90天的发热婴儿中，除了肠道病毒外，还使用分子诊断方法来鉴定旁病毒。此外,这些数据支持在发热≤90天的婴儿中,利用血液标本诊断肠道病毒和parechovirus感染.
我们进行了一项巢式队列研究，通过rtPCR检查15-90天发热婴儿的血浆和CSF样本。对样品进行了呼吸道病毒（呼吸道合胞病毒，流感,肠病毒,副病毒,腺病毒，博卡病毒）。还收集临床和实验室数据以确定严重细菌感染(SBI)的存在。
■24%（126个婴儿中有30个）的血浆/CSF标本对呼吸道病毒呈阳性。肠道病毒和旁病毒是最常见的呼吸道病毒。血浆样品中的病毒阳性率最高，为25％（107个中的27个），而CSF样品为15％（62个中的9个）。与未检测到病毒的婴儿相比，SBI（特别是尿路感染）在呼吸道病毒样本呈阳性的婴儿中不太常见（3％vs.26%,p=0.008)。
■我们的研究结果支持在≤90天的发热婴儿中，除了肠道病毒外，还使用分子诊断方法来鉴定旁病毒。此外,这些数据支持在发热≤90天的婴儿中,利用血液标本诊断肠道病毒和parechovirus感染.
