RESULTS: We found 24 MIPS genes in ten legumes: Arachis hypogea, Cicer arietinum, Cajanus cajan, Glycine max, Lablab purpureus, Medicago truncatula, Pisum sativum, Phaseolus vulgaris, Trifolium pratense and Vigna unguiculata. The total number of MIPS genes found in each species ranged from two to three. The MIPS genes were classified into five clades based on their evolutionary relationships with Arabidopsis genes. The structural patterns of intron/exon and the protein motifs that were conserved in each gene were highly group-specific. In legumes, MIPS genes were inconsistently distributed across their genomes. A comparison of genomes and gene sequences showed that this family was subjected to purifying selection and the gene expansion in MIPS family in legumes was mainly caused by segmental duplication. Through quantitative PCR, expression patterns of MIPS in response to various abiotic stresses, in the vegetative tissues of various legumes were studied. Expression pattern shows that MIPS genes control the development and differentiation of various organs, and have significant responses to salinity and drought stress.
CONCLUSIONS: The MIPS genes in the genomes of legumes have been identified, characterized and their expression was analysed. The findings pave way for understanding their molecular functions and evolution, and lead to identify the putative MIPS genes associated with different cell and tissue development.
结果:我们在10种豆类中发现了24种MIPS基因:Cicerarietinum,Cajanuscajan,最大甘氨酸,Lablabpurpureus,Medicagotruncatula,Pisumsativum,菜豆,白三叶和Vignaunguiculata。在每个物种中发现的MIPS基因的总数为2至3。根据其与拟南芥基因的进化关系,将MIPS基因分为五个分支。内含子/外显子的结构模式和每个基因中保守的蛋白质基序是高度组特异性的。在豆类中,MIPS基因在其基因组中分布不一致。基因组和基因序列的比较表明,该家族经过纯化选择,豆科植物MIPS家族的基因扩增主要是由节段复制引起的。通过定量PCR,响应各种非生物胁迫的MIPS表达模式,在各种豆科植物的营养组织中进行了研究。表达模式表明MIPS基因控制着各器官的发育和分化,对盐分和干旱胁迫有显著响应。
结论:已经鉴定了豆科植物基因组中的MIPS基因,表征并分析了它们的表达。这些发现为理解它们的分子功能和进化铺平了道路,并导致鉴定与不同细胞和组织发育相关的推定MIPS基因。