■葡萄具有很高的经济价值。炭疽病,一种导致葡萄成熟腐烂和叶斑病的病原体,威胁到葡萄的产量和质量。
■本研究通过细胞学研究和转录组测序调查了葡萄树之间的相互作用。
■不同的葡萄种质,五、viniferacv。汤普森无核(TS),V.labrusca登录名Beaumont(B)和V.piasezkiiLiuba-8(LB-8)被归类为高度敏感,中等抗性和抗念珠菌,分别。细胞学研究分析揭示了接种后易感和抗性葡萄之间的明显差异,包括更快的病原体发展,更长的发芽管,念珠菌的正常压疮和易感寄主葡萄中没有白色分泌物。为了解鹿茸的致病机制,对易感葡萄树“TS”进行转录组测序,鉴定了236个差异表达的葡萄念珠菌基因。其中包括56个效应器,36个碳水化合物基因,5个P450基因,和10个参与次生代谢的基因。真菌效应子被认为是调节植物免疫和影响疾病发展的关键致病因子。农杆菌介导的烟草中的瞬时转化筛选了10种效应子(CvA13877,CvA01508,CvA05621,CvA00229,CvA07043,CvA05569,CvA12648,CvA02698,CvA14071和CvA10999),它们抑制了INF1(Cvestelans诱导的细胞死亡。此外,对念珠菌响应的“TS”的转录组分析鉴定出与植物激素信号相关的差异表达的葡萄基因(TGA,PR1,ETR,和ERF1/2),白藜芦醇生物合成基因(STS),苯丙素生物合成基因(PAL和COMT),光合天线蛋白(Lhca和Lhcb),转录因子(WRKY,NAC,MYB,ERF,GATA,bHLH和SBP),ROS(活性氧)清除基因(CAT,GSH,POD和SOD),和疾病相关基因(LRR,RPS2和GST)。
■这项研究强调了葡萄树的潜在功能多样性。本研究结果为进一步研究炭疽菌的侵染机制和鉴定葡萄的疾病反应靶点奠定了基础。
UNASSIGNED: Grape is of high economic value. Colletotrichum viniferum, a pathogen causing grape ripe rot and leaf spot, threatens grape production and quality.
UNASSIGNED: This study investigates the interplay between C. viniferum by Cytological study and transcriptome sequencing.
UNASSIGNED: Different grapevine germplasms, V. vinifera cv. Thompson Seedless (TS), V. labrusca accession Beaumont (B) and V. piasezkii Liuba-8 (LB-8) were classified as highly sensitive, moderate resistant and resistant to C. viniferum, respectively. Cytological study analysis reveals distinct differences between susceptible and resistant grapes post-inoculation, including faster pathogen development, longer germination tubes, normal appressoria of C. viniferum and absence of white secretions in the susceptible host grapevine. To understand the pathogenic mechanisms of C. viniferum, transcriptome sequencing was performed on the susceptible grapevine \"TS\" identifying 236 differentially expressed C. viniferum genes. These included 56 effectors, 36 carbohydrate genes, 5 P450 genes, and 10 genes involved in secondary metabolism. Fungal effectors are known as pivotal pathogenic factors that modulate plant immunity and affect disease development. Agrobacterium-mediated transient transformation in Nicotiana benthamiana screened 10 effectors (CvA13877, CvA01508, CvA05621, CvA00229, CvA07043, CvA05569, CvA12648, CvA02698, CvA14071 and CvA10999) that inhibited INF1 (infestans 1, P. infestans PAMP elicitor) induced cell death and 2 effectors (CvA02641 and CvA11478) that induced cell death. Additionally, transcriptome analysis of \"TS\" in response to C. viniferum identified differentially expressed grape genes related to plant hormone signaling (TGA, PR1, ETR, and ERF1/2), resveratrol biosynthesis genes (STS), phenylpropanoid biosynthesis genes (PAL and COMT), photosynthetic antenna proteins (Lhca and Lhcb), transcription factors (WRKY, NAC, MYB, ERF, GATA, bHLH and SBP), ROS (reactive oxygen species) clearance genes (CAT, GSH, POD and SOD), and disease-related genes (LRR, RPS2 and GST).
UNASSIGNED: This study highlights the potential functional diversity of C. viniferum effectors. Our findings lay a foundation for further research of infection mechanisms in Colletotrichum and identification of disease response targets in grape.