PDF(Pubmed)
Abstract：
BACKGROUND: Epizootic lymphangitis is an infectious and chronically debilitating disease of the equines. Histoplasma capsulatum var. farciminosum, a thermally dimorphic fungi, is the causative agent for the disease. In Ethiopia, the disease significantly affects carthorses, posing threats to animal welfare, and resulting in substantial economic losses. Limited availability of widely accessible antifungals in addition to the chronic nature of the disease is the major challenge against management of epizootic lymphangitis. This study aimed to assess the in vitro efficacy of specific local medicinal plant extracts against the mycelial phase development of H. capsulatum var. farciminosum in southern Ethiopia. The leaves of Xanthium strumarium, Kanda (Family Rubiaceae), Croton macrostachyus (Bisana in Amharic), and Centella Asiatica (Echere waye as a local name in Zeyissegna) that are traditionally used for the treatment of different skin ailments were collected and extracted for the in vitro trial.
RESULTS: The study revealed that methanol extracts of Xanthium strumarium, Kanda, Croton macrostachyus, and Centella Asiatica, at minimum inhibitory concentrations of 1.25 mg/ml, 2.5 mg/ml, 2.5 mg/ml, and 5 mg/ml, respectively, inhibited the growth of H. capsulatum var. farciminosum.
CONCLUSIONS: This in vitro finding could serve as significant preliminary data in the exploration of effective alternative treatment options for epizootic lymphangitis. This study provides a crucial foundation for further research aimed at determining the chemical components and in vivo effectiveness of these plant extracts against both the mycelial and yeast forms of Histoplasma capsulatum var. farciminosum.
RESULTS: The study revealed that methanol extracts of Xanthium strumarium, Kanda, Croton macrostachyus, and Centella Asiatica, at minimum inhibitory concentrations of 1.25 mg/ml, 2.5 mg/ml, 2.5 mg/ml, and 5 mg/ml, respectively, inhibited the growth of H. capsulatum var. farciminosum.
CONCLUSIONS: This in vitro finding could serve as significant preliminary data in the exploration of effective alternative treatment options for epizootic lymphangitis. This study provides a crucial foundation for further research aimed at determining the chemical components and in vivo effectiveness of these plant extracts against both the mycelial and yeast forms of Histoplasma capsulatum var. farciminosum.
摘要：
背景：流行性淋巴管炎是一种传染性和慢性使马匹衰弱的疾病。荚膜组织胞浆变种。Farciminosum,一种热二态真菌，是这种疾病的病原体。在埃塞俄比亚,这种疾病会显著影响carstores，对动物福利构成威胁，造成了巨大的经济损失。除了该疾病的慢性性质外，广泛获得的抗真菌药的有限可用性是针对流行性淋巴管炎管理的主要挑战。本研究旨在评估特定局部药用植物提取物对荚膜H.capsulatumvar菌丝期发育的体外功效。埃塞俄比亚南部的Farciminosum。苍耳的叶子，Kanda（茜草科），Crotonmacrostachyus(BisanainAmharic),收集和提取传统上用于治疗不同皮肤疾病的CentellaAsiatica（Echerewaye为Zeyissegna的本地名称），用于体外试验。
结果：研究表明，苍耳的甲醇提取物，Kanda,Crotonmacrostachyus,和积雪草,在最低抑制浓度为1.25mg/ml时，2.5mg/ml,2.5mg/ml,和5毫克/毫升，分别,抑制荚膜H.Farciminosum.
结论：这一体外发现可作为探索流行性淋巴管炎有效替代治疗方案的重要初步数据。这项研究为进一步研究提供了至关重要的基础，该研究旨在确定这些植物提取物对荚膜组织胞浆菌的菌丝体和酵母形式的化学成分和体内有效性。Farciminosum.
结果：研究表明，苍耳的甲醇提取物，Kanda,Crotonmacrostachyus,和积雪草,在最低抑制浓度为1.25mg/ml时，2.5mg/ml,2.5mg/ml,和5毫克/毫升，分别,抑制荚膜H.Farciminosum.
结论：这一体外发现可作为探索流行性淋巴管炎有效替代治疗方案的重要初步数据。这项研究为进一步研究提供了至关重要的基础，该研究旨在确定这些植物提取物对荚膜组织胞浆菌的菌丝体和酵母形式的化学成分和体内有效性。Farciminosum.
